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Assay Detail

CHEMBL4826012

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of P-gp in human Caco-2 cells assessed as reduction in paclitaxel efflux pre-incubated for 30 mins and measured after 120 mins by LC-MS/MS analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Caco-2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ATP-dependent translocase ABCB1 (CHEMBL4302)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Encequidar, First-in-Class Intestine Specific P-glycoprotein Inhibitor.
Smolinski MP, Urgaonkar S, Pitzonka L, Cutler M, Lee G, Suh KH, Lau JYN.
J Med Chem (2021) 64 : 3677 -3693
PubMed 33729781 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.28 7.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4594298 ENCEQUIDAR 3.0 1 7.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4594298 ENCEQUIDAR IC50 = 53.0 nM 7.28