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Assay Detail

CHEMBL4828545

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]N-methylspiperone from human D2L receptor expressed in HEK293 cell membranes measured after 60 mins by MicroBeta scintillation counting method
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Subcellular Cell membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

D(2) dopamine receptor (CHEMBL217)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure Activity Relationships for a Series of Eticlopride-Based Dopamine D<sub>2</sub>/D<sub>3</sub> Receptor Bitopic Ligands.
Shaik AB, Boateng CA, Battiti FO, Bonifazi A, Cao J, Chen L, Chitsazi R, Ravi S, Lee KH, Shi L, Newman AH.
J Med Chem (2021) 64 : 15313 -15333
PubMed 34636551 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 25 7.81 9.93

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL8946 ETICLOPRIDE 2.0 1 9.93
CHEMBL1112 ARIPIPRAZOLE 4.0 1 9.16
CHEMBL4865434 1 9.16
CHEMBL4846561 1 9.02
CHEMBL4873313 1 8.75
CHEMBL4853568 1 8.73
CHEMBL4847432 1 8.72
CHEMBL4861573 1 8.65
CHEMBL4850300 1 8.64
CHEMBL4860478 1 8.60
CHEMBL4864320 1 8.59
CHEMBL4856983 1 8.14
CHEMBL4868595 1 8.13
CHEMBL4849107 1 7.60
CHEMBL4853938 1 7.56
CHEMBL4871669 1 7.42
CHEMBL4858961 1 7.18
CHEMBL4876353 1 7.13
CHEMBL4864366 1 6.79
CHEMBL4871811 1 6.76
CHEMBL4858824 1 6.69
CHEMBL4875195 1 6.66
CHEMBL4862405 1 5.88
CHEMBL4869151 1 5.75
CHEMBL4868275 1 5.59

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL8946 ETICLOPRIDE Ki = 0.117 nM 9.93
CHEMBL1112 ARIPIPRAZOLE Ki = 0.687 nM 9.16
CHEMBL4865434 Ki = 0.688 nM 9.16
CHEMBL4846561 Ki = 0.947 nM 9.02
CHEMBL4873313 Ki = 1.77 nM 8.75
CHEMBL4853568 Ki = 1.86 nM 8.73
CHEMBL4847432 Ki = 1.9 nM 8.72
CHEMBL4861573 Ki = 2.26 nM 8.65
CHEMBL4850300 Ki = 2.29 nM 8.64
CHEMBL4860478 Ki = 2.54 nM 8.60
CHEMBL4864320 Ki = 2.57 nM 8.59
CHEMBL4856983 Ki = 7.24 nM 8.14
CHEMBL4868595 Ki = 7.42 nM 8.13
CHEMBL4849107 Ki = 25.3 nM 7.60
CHEMBL4853938 Ki = 27.6 nM 7.56
CHEMBL4871669 Ki = 38.2 nM 7.42
CHEMBL4858961 Ki = 66.4 nM 7.18
CHEMBL4876353 Ki = 74.4 nM 7.13
CHEMBL4864366 Ki = 162.0 nM 6.79
CHEMBL4871811 Ki = 173.0 nM 6.76
CHEMBL4858824 Ki = 205.0 nM 6.69
CHEMBL4875195 Ki = 217.0 nM 6.66
CHEMBL4862405 Ki = 1310.0 nM 5.88
CHEMBL4869151 Ki = 1760.0 nM 5.75
CHEMBL4868275 Ki = 2590.0 nM 5.59