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Assay Detail

CHEMBL4829376

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human 20S proteasome LMP2 using Ac-PAL-AMC as fluorogenic substrate measured every minute for 1 hr by fluorescence assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proteasome subunit beta type-9 (CHEMBL1944495)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Macrocyclic Immunoproteasome Inhibitors as a Potential Therapy for Alzheimer's Disease.
Lee MJ, Bhattarai D, Jang H, Baek A, Yeo IJ, Lee S, Miller Z, Lee S, Hong JT, Kim DE, Lee W, Kim KB.
J Med Chem (2021) 64 : 10934 -10950
PubMed 34309393 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 6.54 7.18

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4870740 1 7.18
CHEMBL4867229 1 7.16
CHEMBL4649310 1 7.15
CHEMBL4646371 1 6.98
CHEMBL4879354 1 6.89
CHEMBL4845896 1 6.80
CHEMBL4859824 1 6.80
CHEMBL4875393 1 6.78
CHEMBL4877277 1 6.74
CHEMBL4869559 1 6.62
CHEMBL4864020 1 6.60
CHEMBL4866610 1 6.58
CHEMBL4867231 1 6.38
CHEMBL4863666 1 6.38
CHEMBL4879280 1 6.37
CHEMBL4863407 1 6.28
CHEMBL4863075 1 6.25
CHEMBL4849360 1 5.91
CHEMBL4855585 1 5.67
CHEMBL4854647 1 5.34

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4870740 IC50 = 65.6 nM 7.18
CHEMBL4867229 IC50 = 69.4 nM 7.16
CHEMBL4649310 IC50 = 70.8 nM 7.15
CHEMBL4646371 IC50 = 105.2 nM 6.98
CHEMBL4879354 IC50 = 129.3 nM 6.89
CHEMBL4845896 IC50 = 157.9 nM 6.80
CHEMBL4859824 IC50 = 158.1 nM 6.80
CHEMBL4875393 IC50 = 167.7 nM 6.78
CHEMBL4877277 IC50 = 184.1 nM 6.74
CHEMBL4869559 IC50 = 237.7 nM 6.62
CHEMBL4864020 IC50 = 251.1 nM 6.60
CHEMBL4866610 IC50 = 261.5 nM 6.58
CHEMBL4867231 IC50 = 412.8 nM 6.38
CHEMBL4863666 IC50 = 414.5 nM 6.38
CHEMBL4879280 IC50 = 422.8 nM 6.37
CHEMBL4863407 IC50 = 522.6 nM 6.28
CHEMBL4863075 IC50 = 563.4 nM 6.25
CHEMBL4849360 IC50 = 1245.0 nM 5.91
CHEMBL4855585 IC50 = 2131.0 nM 5.67
CHEMBL4854647 IC50 = 4583.0 nM 5.34