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Assay Detail

CHEMBL4830671

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TNF-alpha stimulated NF-KappaB in human HEK293 cells transfected with NF-kappaB-Luc incubated for 5 hrs by luciferase reporter gene assay
35
Total Activities
35
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Nuclear factor NF-kappa-B complex (CHEMBL2094258)
Type PROTEIN COMPLEX GROUP
Organism Homo sapiens

Publication

Structure-Activity Relationships of Withanolides as Antiproliferative Agents for Multiple Myeloma: Comparison of Activity in 2D Models and a 3D Coculture Model.
Freitas Misakyan MF, Wijeratne EMK, Issa ME, Xu YM, Monteillier A, Gunatilaka AAL, Cuendet M.
J Nat Prod (2021) 84 : 2321 -2335
PubMed 34445874 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 35 6.82 7.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4850374 1 7.82
CHEMBL4848017 1 7.50
CHEMBL4288984 WITHANOLIDE D 3.0 1 7.44
CHEMBL4852942 1 7.39
CHEMBL517080 WITHAFERIN A 1.0 1 7.33
CHEMBL4869263 1 7.30
CHEMBL3234472 1 7.26
CHEMBL3234471 1 7.25
CHEMBL4859862 1 7.23
CHEMBL4857813 1 7.20
CHEMBL2047407 1 7.16
CHEMBL4849546 1 7.15
CHEMBL452952 WITHAPHYSACARPIN 1 7.13
CHEMBL4864258 1 7.10
CHEMBL4870538 1 7.02
CHEMBL4846956 1 7.02
CHEMBL4846709 1 6.99
CHEMBL4847060 1 6.90
CHEMBL4855317 1 6.88
CHEMBL4852605 1 6.84
CHEMBL4851958 1 6.68
CHEMBL4871698 1 6.68
CHEMBL4869516 1 6.66
CHEMBL4855054 1 6.61
CHEMBL4857213 1 6.58
CHEMBL4867721 1 6.56
CHEMBL4863163 1 6.48
CHEMBL4298697 1 6.33
CHEMBL4871909 1 6.32
CHEMBL4282914 1 6.31

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4850374 IC50 = 15.0 nM 7.82
CHEMBL4848017 IC50 = 32.0 nM 7.50
CHEMBL4288984 WITHANOLIDE D IC50 = 36.0 nM 7.44
CHEMBL4852942 IC50 = 41.0 nM 7.39
CHEMBL517080 WITHAFERIN A IC50 = 47.0 nM 7.33
CHEMBL4869263 IC50 = 50.0 nM 7.30
CHEMBL3234472 IC50 = 55.0 nM 7.26
CHEMBL3234471 IC50 = 56.0 nM 7.25
CHEMBL4859862 IC50 = 59.0 nM 7.23
CHEMBL4857813 IC50 = 63.0 nM 7.20
CHEMBL2047407 IC50 = 70.0 nM 7.16
CHEMBL4849546 IC50 = 71.0 nM 7.15
CHEMBL452952 WITHAPHYSACARPIN IC50 = 74.0 nM 7.13
CHEMBL4864258 IC50 = 80.0 nM 7.10
CHEMBL4846956 IC50 = 95.0 nM 7.02
CHEMBL4870538 IC50 = 95.0 nM 7.02
CHEMBL4846709 IC50 = 102.0 nM 6.99
CHEMBL4847060 IC50 = 127.0 nM 6.90
CHEMBL4855317 IC50 = 133.0 nM 6.88
CHEMBL4852605 IC50 = 146.0 nM 6.84
CHEMBL4851958 IC50 = 209.0 nM 6.68
CHEMBL4871698 IC50 = 211.0 nM 6.68
CHEMBL4869516 IC50 = 220.0 nM 6.66
CHEMBL4855054 IC50 = 245.0 nM 6.61
CHEMBL4857213 IC50 = 261.0 nM 6.58
CHEMBL4867721 IC50 = 276.0 nM 6.56
CHEMBL4863163 IC50 = 332.0 nM 6.48
CHEMBL4298697 IC50 = 467.0 nM 6.33
CHEMBL4871909 IC50 = 480.0 nM 6.32
CHEMBL4282914 IC50 = 493.0 nM 6.31
CHEMBL4861753 IC50 = 841.0 nM 6.08
CHEMBL469945 IC50 = 913.0 nM 6.04
CHEMBL4852603 IC50 = 1075.0 nM 5.97
CHEMBL4864781 IC50 = 2093.0 nM 5.68
CHEMBL4854305 IC50 = 2093.0 nM 5.68