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Assay Detail

CHEMBL4831996

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant N-terminal His-tagged GSK3beta expressed in Escherichia coli using RRRPASVPPSPSLS RHS(pS)HQRR as substrate incubated for 30 mins in presence of ATP by Kinase-Glo reagent based luminescence assay
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glycogen synthase kinase-3 beta (CHEMBL262)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 2-(cyclopropanecarboxamido)-N-(5-((1-(4-fluorobenzyl)piperidin-4-yl)methoxy)pyridin-3-yl)isonicotinamide as a potent dual AChE/GSK3β inhibitor for the treatment of Alzheimer's disease: Significantly increasing the level of acetylcholine in the brain without affecting that in intestine.
Jiang X, Liu C, Zou M, Xie H, Lin T, Lyu W, Xu J, Li Y, Feng F, Sun H, Liu W.
Eur J Med Chem (2021) 223 : 113663 -113663
PubMed 34198150 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 6.63 7.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4863960 1 7.64
CHEMBL102714 SB-216763 1 7.54
CHEMBL4873612 1 7.51
CHEMBL4854209 1 7.20
CHEMBL4865061 1 7.11
CHEMBL4854572 1 7.10
CHEMBL4867249 1 7.10
CHEMBL4857955 1 7.09
CHEMBL4873162 1 7.02
CHEMBL4866150 1 7.01
CHEMBL4857680 1 6.90
CHEMBL4853675 1 6.82
CHEMBL4872690 1 6.80
CHEMBL4870746 1 6.79
CHEMBL4851865 1 6.70
CHEMBL4864059 1 6.64
CHEMBL4868956 1 6.61
CHEMBL4865615 1 6.55
CHEMBL4851835 1 6.52
CHEMBL4855714 1 6.13
CHEMBL4877175 1 6.08
CHEMBL4875903 1 5.46
CHEMBL4849828 1 5.31
CHEMBL4862811 1 5.17
CHEMBL4855201 1 5.01
CHEMBL4846646 1 -
CHEMBL4870273 1 -
CHEMBL4846945 1 -
CHEMBL4877556 1 -
CHEMBL4850776 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4863960 IC50 = 23.0 nM 7.64
CHEMBL102714 SB-216763 IC50 = 29.0 nM 7.54
CHEMBL4873612 IC50 = 31.0 nM 7.51
CHEMBL4854209 IC50 = 63.0 nM 7.20
CHEMBL4865061 IC50 = 77.0 nM 7.11
CHEMBL4854572 IC50 = 79.0 nM 7.10
CHEMBL4867249 IC50 = 79.0 nM 7.10
CHEMBL4857955 IC50 = 81.0 nM 7.09
CHEMBL4873162 IC50 = 95.0 nM 7.02
CHEMBL4866150 IC50 = 97.0 nM 7.01
CHEMBL4857680 IC50 = 126.0 nM 6.90
CHEMBL4853675 IC50 = 152.0 nM 6.82
CHEMBL4872690 IC50 = 157.0 nM 6.80
CHEMBL4870746 IC50 = 164.0 nM 6.79
CHEMBL4851865 IC50 = 198.0 nM 6.70
CHEMBL4864059 IC50 = 231.0 nM 6.64
CHEMBL4868956 IC50 = 244.0 nM 6.61
CHEMBL4865615 IC50 = 279.0 nM 6.55
CHEMBL4851835 IC50 = 305.0 nM 6.52
CHEMBL4855714 IC50 = 734.0 nM 6.13
CHEMBL4877175 IC50 = 837.0 nM 6.08
CHEMBL4875903 IC50 = 3508.0 nM 5.46
CHEMBL4849828 IC50 = 4935.0 nM 5.31
CHEMBL4862811 IC50 = 6804.0 nM 5.17
CHEMBL4855201 IC50 = 9748.0 nM 5.01
CHEMBL4846646 IC50 > 10000.0 nM -
CHEMBL4870273 IC50 > 10000.0 nM -
CHEMBL4846945 IC50 > 10000.0 nM -
CHEMBL4877556 IC50 > 10000.0 nM -
CHEMBL4850776 IC50 > 10000.0 nM -
CHEMBL4870877 IC50 > 10000.0 nM -
CHEMBL4859041 IC50 > 10000.0 nM -
CHEMBL502 DONEPEZIL IC50 > 10000.0 nM -