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Assay Detail

CHEMBL4833336

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to 6His-tagged HMGB1 (unknown origin) incubated for 15 mins by MST analysis
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

High mobility group protein B1 (CHEMBL2311236)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Systematic Development of Peptide Inhibitors Targeting the CXCL12/HMGB1 Interaction.
Sgrignani J, Cecchinato V, Fassi EMA, D'Agostino G, Garofalo M, Danelon G, Pedotti M, Simonelli L, Varani L, Grazioso G, Uguccioni M, Cavalli A.
J Med Chem (2021) 64 : 13439 -13450
PubMed 34510899 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 13 5.26 6.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4867183 1 6.10
CHEMBL4875121 1 6.10
CHEMBL4858278 1 5.72
CHEMBL4860199 1 5.24
CHEMBL4858896 1 5.07
CHEMBL4850898 1 5.00
CHEMBL4853693 1 4.85
CHEMBL4853073 1 4.66
CHEMBL4864623 1 4.58
CHEMBL4857636 1 -
CHEMBL4859744 1 -
CHEMBL4851154 1 -
CHEMBL4858354 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4867183 Kd = 800.0 nM 6.10
CHEMBL4875121 Kd = 800.0 nM 6.10
CHEMBL4858278 Kd = 1900.0 nM 5.72
CHEMBL4860199 Kd = 5800.0 nM 5.24
CHEMBL4858896 Kd = 8600.0 nM 5.07
CHEMBL4850898 Kd = 9900.0 nM 5.00
CHEMBL4853693 Kd = 14000.0 nM 4.85
CHEMBL4853073 Kd = 22000.0 nM 4.66
CHEMBL4864623 Kd = 26200.0 nM 4.58
CHEMBL4857636 Kd - -
CHEMBL4859744 Kd > 80000.0 nM -
CHEMBL4851154 Kd - -
CHEMBL4858354 Kd = 160000.0 nM -