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Assay Detail

CHEMBL4833429

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SARS-CoV-2 3CL protease using HiyteFluor-488ESATL-QSGLRKAK-(QXL)-NH2 fluorogenic peptide as substrate preincubated for 15 mins followed by substrate addition and measured after 30 mins by FRET assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Indole Chloropyridinyl Ester-Derived SARS-CoV-2 3CLpro Inhibitors: Enzyme Inhibition, Antiviral Efficacy, Structure-Activity Relationship, and X-ray Structural Studies.
Ghosh AK, Raghavaiah J, Shahabi D, Yadav M, Anson BJ, Lendy EK, Hattori SI, Higashi-Kuwata N, Mitsuya H, Mesecar AD.
J Med Chem (2021) 64 : 14702 -14714
PubMed 34528437 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 5.93 7.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4852507 1 7.14
CHEMBL496316 1 6.92
CHEMBL490820 1 6.60
CHEMBL510559 1 6.51
CHEMBL4874639 1 6.50
CHEMBL4866309 1 6.47
CHEMBL4858401 1 6.42
CHEMBL4860159 1 6.38
CHEMBL4879042 1 6.33
CHEMBL4848976 1 6.31
CHEMBL4853474 1 6.23
CHEMBL4846171 1 6.06
CHEMBL4853263 1 6.05
CHEMBL4875182 1 5.92
CHEMBL4862535 1 5.66
CHEMBL4857985 1 5.36
CHEMBL4864512 1 4.99
CHEMBL4872700 1 4.82
CHEMBL4864365 1 4.00
CHEMBL4874041 1 4.00
CHEMBL4859897 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4852507 IC50 = 73.0 nM 7.14
CHEMBL496316 IC50 = 120.0 nM 6.92
CHEMBL490820 IC50 = 250.0 nM 6.60
CHEMBL510559 IC50 = 310.0 nM 6.51
CHEMBL4874639 IC50 = 320.0 nM 6.50
CHEMBL4866309 IC50 = 340.0 nM 6.47
CHEMBL4858401 IC50 = 380.0 nM 6.42
CHEMBL4860159 IC50 = 420.0 nM 6.38
CHEMBL4879042 IC50 = 470.0 nM 6.33
CHEMBL4848976 IC50 = 490.0 nM 6.31
CHEMBL4853474 IC50 = 590.0 nM 6.23
CHEMBL4846171 IC50 = 870.0 nM 6.06
CHEMBL4853263 IC50 = 900.0 nM 6.05
CHEMBL4875182 IC50 = 1190.0 nM 5.92
CHEMBL4862535 IC50 = 2200.0 nM 5.66
CHEMBL4857985 IC50 = 4400.0 nM 5.36
CHEMBL4864512 IC50 = 10300.0 nM 4.99
CHEMBL4872700 IC50 = 15300.0 nM 4.82
CHEMBL4864365 IC50 = 100000.0 nM 4.00
CHEMBL4874041 IC50 = 100000.0 nM 4.00
CHEMBL4859897 IC50 > 100000.0 nM -