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Assay Detail

CHEMBL4835176

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant MMP2 (AA34 to 660) assessed as cleavage of fluorescent substrate using 390 MMP FRET as substrate incubated for 30 mins by biochemical assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

72 kDa type IV collagenase (CHEMBL333)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of GLPG1972/S201086, a Potent, Selective, and Orally Bioavailable ADAMTS-5 Inhibitor for the Treatment of Osteoarthritis.
Brebion F, Gosmini R, Deprez P, Varin M, Peixoto C, Alvey L, Jary H, Bienvenu N, Triballeau N, Blanque R, Cottereaux C, Christophe T, Vandervoort N, Mollat P, Touitou R, Leonard P, De Ceuninck F, Botez I, Monjardet A, van der Aar E, Amantini D.
J Med Chem (2021) 64 : 2937 -2952
PubMed 33719441 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 5.86 6.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4877087 1 6.48
CHEMBL4862188 1 6.20
CHEMBL4650334 ALDUMASTAT 2.0 1 5.94
CHEMBL4846457 1 5.55
CHEMBL4876618 1 5.51
CHEMBL4877662 1 5.47
CHEMBL4854306 1 -
CHEMBL4874226 1 -
CHEMBL4863614 1 -
CHEMBL4856883 1 -
CHEMBL4876864 1 -
CHEMBL4856943 1 -
CHEMBL4868710 1 -
CHEMBL4873384 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4877087 IC50 = 328.0 nM 6.48
CHEMBL4862188 IC50 = 631.0 nM 6.20
CHEMBL4650334 ALDUMASTAT IC50 = 1158.0 nM 5.94
CHEMBL4846457 IC50 = 2796.0 nM 5.55
CHEMBL4876618 IC50 = 3113.0 nM 5.51
CHEMBL4877662 IC50 = 3382.0 nM 5.47
CHEMBL4854306 IC50 > 4000.0 nM -
CHEMBL4874226 IC50 > 20000.0 nM -
CHEMBL4863614 IC50 > 20000.0 nM -
CHEMBL4856883 IC50 > 4000.0 nM -
CHEMBL4876864 IC50 > 4000.0 nM -
CHEMBL4856943 IC50 > 4000.0 nM -
CHEMBL4868710 IC50 > 4000.0 nM -
CHEMBL4873384 IC50 > 20000.0 nM -