Assay Detail
Binding
CHEMBL4836606
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of MTase activity of SARS-CoV-1 WHU NSP14 expressed in Escherichia coli BL21(DE3) using GpppA and S-adenosyl-[methyl-3H]methionine as substrate preincubated with enzyme for 20 mins followed by S-adenosyl-[methyl-3H]methionine addition and measured after 1.5 hrs by scintillation counting analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Severe acute respiratory syndrome-related coronavirus |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Potent SARS-CoV-2 mRNA Cap Methyltransferase Inhibitors by Bioisosteric Replacement of Methionine in SAM Cosubstrate.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 6.42 | 6.42 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1214186 | SINEFUNGIN | 2.0 | 1 | 6.42 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1214186 | SINEFUNGIN | IC50 | = | 383.0 | nM | 6.42 |