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Assay Detail

CHEMBL4838604

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]NO711 binding to mouse GAT1 expressed in human HEK293 cell line by MS binding assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium- and chloride-dependent GABA transporter 1 (CHEMBL5445)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Development of tricyclic N-benzyl-4-hydroxybutanamide derivatives as inhibitors of GABA transporters mGAT1-4 with anticonvulsant, antinociceptive, and antidepressant activity.
Zaręba P, Sałat K, Höfner GC, Łątka K, Bajda M, Latacz G, Kotniewicz K, Rapacz A, Podkowa A, Maj M, Jóźwiak K, Filipek B, Wanner KT, Malawska B, Kulig K.
Eur J Med Chem (2021) 221 : 113512 -113512
PubMed 34015586 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.63 8.29

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4595698 1 8.29
CHEMBL1027 TIAGABINE 4.0 1 7.43
CHEMBL4861574 1 5.14
CHEMBL4852705 1 4.96
CHEMBL3289575 1 4.96
CHEMBL4859850 1 4.87
CHEMBL4854550 1 4.78
CHEMBL4873878 1 4.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4595698 IC50 = 5.129 nM 8.29
CHEMBL1027 TIAGABINE IC50 = 37.15 nM 7.43
CHEMBL4861574 IC50 = 7244.36 nM 5.14
CHEMBL4852705 IC50 = 10964.78 nM 4.96
CHEMBL3289575 IC50 = 10964.78 nM 4.96
CHEMBL4859850 IC50 = 13489.63 nM 4.87
CHEMBL4854550 IC50 = 16595.87 nM 4.78
CHEMBL4873878 IC50 = 25118.86 nM 4.60