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Assay Detail

CHEMBL4838840

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Influenza A virus (A/California/04/2009 (H1N1)) Neuraminidase H274Y mutant using MUNANA as substrate preincubated for 5 to 15 mins followed by substrate addition and measured after 30 mins by fluorescence spectrophotometric method
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Influenza A virus (A/California/04/2009(H1N1))
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Neuraminidase (CHEMBL3559643)
Type SINGLE PROTEIN
Organism Influenza A virus (strain A/USSR/90/1977 H1N1)

Publication

Discovery of hydrazide-containing oseltamivir analogues as potent inhibitors of influenza A neuraminidase.
Zhao H, Jiang S, Ye Z, Zhu H, Hu B, Meng P, Hu Y, Zhang H, Wang K, Wang J, Tian Y.
Eur J Med Chem (2021) 221 : 113567 -113567
PubMed 34082224 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.55 6.93

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4860142 1 6.93
CHEMBL4867418 1 6.88
CHEMBL4863905 1 6.83
CHEMBL4873884 1 6.42
CHEMBL4797391 1 6.18
CHEMBL674 OSELTAMIVIR CARBOXYLATE -1.0 1 6.08

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4860142 IC50 = 118.0 nM 6.93
CHEMBL4867418 IC50 = 133.0 nM 6.88
CHEMBL4863905 IC50 = 147.0 nM 6.83
CHEMBL4873884 IC50 = 384.0 nM 6.42
CHEMBL4797391 IC50 = 667.0 nM 6.18
CHEMBL674 OSELTAMIVIR CARBOXYLATE IC50 = 828.0 nM 6.08