Assay Detail
Binding
CHEMBL4841801
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human myc-TNNI3K expressed in HEK-MSR2 cells assessed as reduction in TNNI3K autophosphorylation preincubated for 30 mins followed by pervanadate addition and measured after 20 mins by DELFIA
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Serine/threonine-protein kinase TNNI3K (CHEMBL5260) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Identification of Diarylurea Inhibitors of the Cardiac-Specific Kinase TNNI3K by Designing Selectivity Against VEGFR2, p38α, and B-Raf.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 11 | 7.39 | 8.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4868757 | — | — | 1 | 8.10 |
| CHEMBL4094739 | — | — | 1 | 8.10 |
| CHEMBL4849349 | — | — | 1 | 7.80 |
| CHEMBL4863580 | — | — | 1 | 7.70 |
| CHEMBL4871527 | — | — | 1 | 7.40 |
| CHEMBL4854957 | — | — | 1 | 7.30 |
| CHEMBL3613195 | — | — | 1 | 7.30 |
| CHEMBL4869303 | — | — | 1 | 7.00 |
| CHEMBL4867552 | — | — | 1 | 7.00 |
| CHEMBL3815093 | — | — | 1 | 7.00 |
| CHEMBL1336 | SORAFENIB | 4.0 | 1 | 6.60 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4868757 | — | IC50 | = | 7.9 | nM | 8.10 |
| CHEMBL4094739 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL4849349 | — | IC50 | = | 16.0 | nM | 7.80 |
| CHEMBL4863580 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL4871527 | — | IC50 | = | 40.0 | nM | 7.40 |
| CHEMBL4854957 | — | IC50 | = | 50.0 | nM | 7.30 |
| CHEMBL3613195 | — | IC50 | = | 50.0 | nM | 7.30 |
| CHEMBL4869303 | — | IC50 | = | 100.0 | nM | 7.00 |
| CHEMBL4867552 | — | IC50 | = | 100.0 | nM | 7.00 |
| CHEMBL3815093 | — | IC50 | = | 100.0 | nM | 7.00 |
| CHEMBL1336 | SORAFENIB | IC50 | = | 250.0 | nM | 6.60 |