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Assay Detail

CHEMBL4842434

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Non-competitive inhibition of human LDHA assessed as reduction in lactate production using pyruvate as substrate in presence of NADH by Lineweaver-Burk plot analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

L-lactate dehydrogenase A chain (CHEMBL4835)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Cannabichromene and Δ<sup>9</sup>-Tetrahydrocannabinolic Acid Identified as Lactate Dehydrogenase-A Inhibitors by <i>in Silico</i> and <i>in Vitro</i> Screening.
Martin LJ, Cairns EA, Heblinski M, Fletcher C, Krycer JR, Arnold JC, McGregor IS, Bowen MT, Anderson LL.
J Nat Prod (2021) 84 : 1469 -1477
PubMed 33887133 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 4.93 5.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL465718 DELTA9-TETRAHYDROCANNABINOLIC ACID A 1 5.19
CHEMBL422704 CANNABICHROMENE 1 5.07
CHEMBL465 DRONABINOL 4.0 1 4.85
CHEMBL190461 CANNABIDIOL 4.0 1 4.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL465718 DELTA9-TETRAHYDROCANNABINOLIC ACID A Ki = 6500.0 nM 5.19
CHEMBL422704 CANNABICHROMENE Ki = 8500.0 nM 5.07
CHEMBL465 DRONABINOL Ki = 14000.0 nM 4.85
CHEMBL190461 CANNABIDIOL Ki = 24000.0 nM 4.62