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Assay Detail

CHEMBL4842515

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Anti-necroptotic activity in human HT-29 cells assessed as inhibition of hTNFalpha/Z-VAD-fmk (TSZ)-induced necroptosis by measuring increase in cell viability measured after 8 to 10 hrs by celltiter-glo luminescent cell viability assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 1 — Organism
Curated By Autocuration

Target

HT-29 (CHEMBL384)
Type CELL-LINE
Organism Homo sapiens

Publication

Structure-based bioisosterism design of thio-benzoxazepinones as novel necroptosis inhibitors.
Xia C, Yao Z, Xu L, Zhang W, Chen H, Zhuang C.
Eur J Med Chem (2021) 220 : 113484 -113484
PubMed 33930803 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 27 6.72 8.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4871408 1 8.55
CHEMBL4071864 GSK2982772 2.0 1 8.44
CHEMBL4850691 1 8.13
CHEMBL4866295 1 7.86
CHEMBL4861471 ECLITASERTIB 2.0 1 7.81
CHEMBL4866261 1 7.73
CHEMBL4872011 1 7.65
CHEMBL4848275 1 7.12
CHEMBL4874864 1 6.91
CHEMBL4860261 1 6.84
CHEMBL4849815 1 6.67
CHEMBL4874139 1 6.61
CHEMBL4090420 1 6.55
CHEMBL4872802 1 6.44
CHEMBL4861390 1 6.13
CHEMBL4853355 1 6.09
CHEMBL4865111 1 5.82
CHEMBL4863402 1 5.70
CHEMBL4869067 1 5.47
CHEMBL4873040 1 5.19
CHEMBL4864822 1 5.17
CHEMBL4854997 1 5.05
CHEMBL4871037 1 -
CHEMBL4855090 1 -
CHEMBL4870503 1 -
CHEMBL4847972 1 -
CHEMBL4859911 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4871408 EC50 = 2.8 nM 8.55
CHEMBL4071864 GSK2982772 EC50 = 3.6 nM 8.44
CHEMBL4850691 EC50 = 7.4 nM 8.13
CHEMBL4866295 EC50 = 13.7 nM 7.86
CHEMBL4861471 ECLITASERTIB EC50 = 15.4 nM 7.81
CHEMBL4866261 EC50 = 18.6 nM 7.73
CHEMBL4872011 EC50 = 22.6 nM 7.65
CHEMBL4848275 EC50 = 76.3 nM 7.12
CHEMBL4874864 EC50 = 121.8 nM 6.91
CHEMBL4860261 EC50 = 143.8 nM 6.84
CHEMBL4849815 EC50 = 211.2 nM 6.67
CHEMBL4874139 EC50 = 243.0 nM 6.61
CHEMBL4090420 EC50 = 279.2 nM 6.55
CHEMBL4872802 EC50 = 363.0 nM 6.44
CHEMBL4861390 EC50 = 735.6 nM 6.13
CHEMBL4853355 EC50 = 810.5 nM 6.09
CHEMBL4865111 EC50 = 1513.0 nM 5.82
CHEMBL4863402 EC50 = 2017.5 nM 5.70
CHEMBL4869067 EC50 = 3402.5 nM 5.47
CHEMBL4873040 EC50 = 6496.0 nM 5.19
CHEMBL4864822 EC50 = 6677.0 nM 5.17
CHEMBL4854997 EC50 = 8837.0 nM 5.05
CHEMBL4871037 EC50 > 10000.0 nM -
CHEMBL4855090 EC50 > 10000.0 nM -
CHEMBL4870503 EC50 > 10000.0 nM -
CHEMBL4847972 EC50 > 10000.0 nM -
CHEMBL4859911 EC50 > 10000.0 nM -