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Assay Detail

CHEMBL4843955

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant HDAC8 using H2N-Arg-His-Lys(Ac)-Lys(Ac)-AMC as substrate after 90 mins by fluorescence based micro plate assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 8 (CHEMBL3192)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis, structure-activity relationships, cocrystallization and cellular characterization of novel smHDAC8 inhibitors for the treatment of schistosomiasis.
Ghazy E, Heimburg T, Lancelot J, Zeyen P, Schmidtkunz K, Truhn A, Darwish S, Simoben CV, Shaik TB, Erdmann F, Schmidt M, Robaa D, Romier C, Jung M, Pierce R, Sippl W.
Eur J Med Chem (2021) 225 : 113745 -113745
PubMed 34392190 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 6.72 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2170177 1 8.00
CHEMBL4095643 1 7.40
CHEMBL4868510 1 7.16
CHEMBL4846424 1 7.05
CHEMBL4866890 1 7.05
CHEMBL4865911 1 7.00
CHEMBL4065863 1 6.96
CHEMBL3800394 1 6.89
CHEMBL4852163 1 6.85
CHEMBL4877604 1 6.82
CHEMBL4867661 1 6.75
CHEMBL4093480 1 6.75
CHEMBL4870162 1 6.66
CHEMBL4853162 1 6.66
CHEMBL4867041 1 6.64
CHEMBL4847732 1 6.57
CHEMBL4850958 1 6.55
CHEMBL4853828 1 6.54
CHEMBL4847326 1 6.50
CHEMBL98 VORINOSTAT 4.0 1 6.40
CHEMBL4857763 1 6.35
CHEMBL4870946 1 6.23
CHEMBL4849600 1 6.11
CHEMBL4869895 1 6.07
CHEMBL4855490 1 5.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2170177 IC50 = 10.0 nM 8.00
CHEMBL4095643 IC50 = 40.0 nM 7.40
CHEMBL4868510 IC50 = 70.0 nM 7.16
CHEMBL4846424 IC50 = 90.0 nM 7.05
CHEMBL4866890 IC50 = 90.0 nM 7.05
CHEMBL4865911 IC50 = 100.0 nM 7.00
CHEMBL4065863 IC50 = 110.0 nM 6.96
CHEMBL3800394 IC50 = 130.0 nM 6.89
CHEMBL4852163 IC50 = 140.0 nM 6.85
CHEMBL4877604 IC50 = 150.0 nM 6.82
CHEMBL4867661 IC50 = 180.0 nM 6.75
CHEMBL4093480 IC50 = 180.0 nM 6.75
CHEMBL4870162 IC50 = 220.0 nM 6.66
CHEMBL4853162 IC50 = 220.0 nM 6.66
CHEMBL4867041 IC50 = 230.0 nM 6.64
CHEMBL4847732 IC50 = 270.0 nM 6.57
CHEMBL4850958 IC50 = 280.0 nM 6.55
CHEMBL4853828 IC50 = 290.0 nM 6.54
CHEMBL4847326 IC50 = 320.0 nM 6.50
CHEMBL98 VORINOSTAT IC50 = 400.0 nM 6.40
CHEMBL4857763 IC50 = 450.0 nM 6.35
CHEMBL4870946 IC50 = 590.0 nM 6.23
CHEMBL4849600 IC50 = 780.0 nM 6.11
CHEMBL4869895 IC50 = 860.0 nM 6.07
CHEMBL4855490 IC50 = 1100.0 nM 5.96