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Assay Detail

CHEMBL4844538

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human 5-HT3 receptor expressed in CHO-K1 cells assessed as inhibition of 5-HT induced inward currents preincubated for 30 sec followed by 5HT addition for 2 sec at -60 mV holding potential by ion flux automated patch clamp assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 3A (CHEMBL1899)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Design and Optimization of FPPQ, a Dual-Acting 5-HT<sub>3</sub> and 5-HT<sub>6</sub> Receptor Antagonist with Antipsychotic and Procognitive Properties.
Zajdel P, Grychowska K, Mogilski S, Kurczab R, Satała G, Bugno R, Kos T, Gołębiowska J, Malikowska-Racia N, Nikiforuk A, Chaumont-Dubel S, Bantreil X, Pawłowski M, Martinez J, Subra G, Lamaty F, Marin P, Bojarski AJ, Popik P.
J Med Chem (2021) 64 : 13279 -13298
PubMed 34467765 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.97 8.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1189679 PALONOSETRON 4.0 1 8.77
CHEMBL4867565 1 7.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1189679 PALONOSETRON IC50 = 1.7 nM 8.77
CHEMBL4867565 IC50 = 67.6 nM 7.17