Assay Detail
Binding
CHEMBL4883004
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Protein Serine/Threonine Kinase, MAPK14 (p38α) Eurofins-Panlabs enzyme assay
8
Total Activities
4
Compounds Tested
2
Activity Types
8
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Profiling data from Eurofins-Panlabs
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Inhibition | 7 | - | - |
| IC50 | 1 | 8.53 | 8.53 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| Assay protocol publication | Assay protocol publication | - | — | PMID: 9598985 |
| Detection method | Detection method | - | — | ELISA quantitation of MBP-P |
| Incubation temperature | Incubation temperature | - | — | 25 °C |
| Incubation time | Incubation time | - | — | 60 min |
| Reference compound | Reference compound | - | — | SB-Z0Z190 |
| reference compound EC50 | reference compound EC50 | 0.015 | uM | — |
| Source of protein | Source of protein | - | — | Human recombinant E. coli |
| Substrate with concentration | Substrate with concentration | - | — | 10 µg/mL Myelin Basic Protein (MBP) |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1800685 | PPTN | — | 5 | 8.53 |
| CHEMBL2204538 | THPP-1 | — | 1 | - |
| CHEMBL1800285 | MSD-M1PAM | — | 1 | - |
| CHEMBL2048623 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1800685 | PPTN | IC50 | = | 2.94 | nM | 8.53 |
| CHEMBL1800685 | PPTN | Inhibition | = | -76.0 | % | - |
| CHEMBL1800685 | PPTN | Inhibition | = | -7.0 | % | - |
| CHEMBL1800685 | PPTN | Inhibition | = | - | % | - |
| CHEMBL1800685 | PPTN | Inhibition | = | 9.0 | % | - |
| CHEMBL2204538 | THPP-1 | Inhibition | = | -14.0 | % | - |
| CHEMBL1800285 | MSD-M1PAM | Inhibition | = | -3.0 | % | - |
| CHEMBL2048623 | — | Inhibition | = | 60.0 | % | - |