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Assay Detail

CHEMBL5029741

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BACE2 (unknown origin) using APP harboring Swedish Lys-Met/Asn-Leu mutant-derived peptide as substrate incubated for 2 hrs by FRET assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-secretase 2 (CHEMBL2525)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

JNJ-67569762, A 2-Aminotetrahydropyridine-Based Selective BACE1 Inhibitor Targeting the S3 Pocket: From Discovery to Clinical Candidate.
Rombouts FJR, Kusakabe KI, Alexander R, Austin N, Borghys H, De Cleyn M, Dhuyvetter D, Gijsen HJM, Hrupka B, Jacobs T, Jerhaoui S, Lammens L, Leclercq L, Tsubone K, Ueno T, Morimoto K, Einaru S, Sumiyoshi H, Van den Bergh A, Vos A, Surkyn M, Teisman A, Moechars D.
J Med Chem (2021) 64 : 14175 -14191
PubMed 34553934 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 6.74 8.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5074719 1 8.51
CHEMBL5081571 1 7.80
CHEMBL5093088 1 7.73
CHEMBL5084928 1 7.33
CHEMBL5091877 1 7.18
CHEMBL5090362 1 6.83
CHEMBL5082356 1 6.72
CHEMBL5092328 1 6.71
CHEMBL5074678 1 6.47
CHEMBL5083750 1 6.39
CHEMBL5089679 1 6.33
CHEMBL5075689 1 6.32
CHEMBL5075795 1 5.94
CHEMBL4465534 1 5.68
CHEMBL5088971 1 5.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5074719 IC50 = 3.1 nM 8.51
CHEMBL5081571 IC50 = 16.0 nM 7.80
CHEMBL5093088 IC50 = 18.6 nM 7.73
CHEMBL5084928 IC50 = 47.0 nM 7.33
CHEMBL5091877 IC50 = 66.0 nM 7.18
CHEMBL5090362 IC50 = 148.0 nM 6.83
CHEMBL5082356 IC50 = 189.0 nM 6.72
CHEMBL5092328 IC50 = 195.0 nM 6.71
CHEMBL5074678 IC50 = 340.0 nM 6.47
CHEMBL5083750 IC50 = 407.0 nM 6.39
CHEMBL5089679 IC50 = 468.0 nM 6.33
CHEMBL5075689 IC50 = 479.0 nM 6.32
CHEMBL5075795 IC50 = 1148.0 nM 5.94
CHEMBL4465534 IC50 = 2100.0 nM 5.68
CHEMBL5088971 IC50 = 6700.0 nM 5.17