Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5031862

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H] JNJ-962 from BACE2 (unknown origin) expressed in HEK293 cell membranes assessed as inhibition constant at pH 6.2 by scintillation counting analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-secretase 2 (CHEMBL2525)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Extremely Selective Fused Pyridine-Derived β-Site Amyloid Precursor Protein-Cleaving Enzyme (BACE1) Inhibitors with High In Vivo Efficacy through 10s Loop Interactions.
Ueno T, Matsuoka E, Asada N, Yamamoto S, Kanegawa N, Ito M, Ito H, Moechars D, Rombouts FJR, Gijsen HJM, Kusakabe KI.
J Med Chem (2021) 64 : 14165 -14174
PubMed 34553947 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 8.16 9.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4557670 1 9.92
CHEMBL5075689 1 8.02
CHEMBL3301601 VERUBECESTAT 3.0 1 7.96
CHEMBL3916243 ATABECESTAT 2.0 1 6.73

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4557670 Ki = 0.12 nM 9.92
CHEMBL5075689 Ki = 9.6 nM 8.02
CHEMBL3301601 VERUBECESTAT Ki = 10.9 nM 7.96
CHEMBL3916243 ATABECESTAT Ki = 185.0 nM 6.73