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Assay Detail

CHEMBL5034076

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Binding
Binding affinity to Bacillus subtilis FtsZ polymer assessed as dissociation constant by fluorescence anisotropy analysis
18
Total Activities
18
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bacillus subtilis
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cell division protein FtsZ (CHEMBL2417349)
Type SINGLE PROTEIN
Organism Bacillus subtilis

Publication

Targeting the FtsZ Allosteric Binding Site with a Novel Fluorescence Polarization Screen, Cytological and Structural Approaches for Antibacterial Discovery.
Huecas S, Araújo-Bazán L, Ruiz FM, Ruiz-Ávila LB, Martínez RF, Escobar-Peña A, Artola M, Vázquez-Villa H, Martín-Fontecha M, Fernández-Tornero C, López-Rodríguez ML, Andreu JM.
J Med Chem (2021) 64 : 5730 -5745
PubMed 33908781 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 17 5.44 6.70
Activity 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5085404 1 6.70
CHEMBL5080731 1 6.16
CHEMBL5076408 1 5.96
CHEMBL5092442 1 5.89
CHEMBL5085966 1 5.82
CHEMBL5074266 1 5.72
CHEMBL5076150 1 5.72
CHEMBL5089927 1 5.42
CHEMBL5090219 1 5.23
CHEMBL5081697 1 5.17
CHEMBL5092196 1 5.15
CHEMBL5090793 1 5.10
CHEMBL5076899 1 5.03
CHEMBL5079700 1 5.02
CHEMBL5081559 1 4.82
CHEMBL5078275 1 4.15
CHEMBL511201 1 -
CHEMBL5091172 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5085404 Kd = 200.0 nM 6.70
CHEMBL5080731 Kd = 700.0 nM 6.16
CHEMBL5076408 Kd = 1100.0 nM 5.96
CHEMBL5092442 Kd = 1300.0 nM 5.89
CHEMBL5085966 Kd = 1500.0 nM 5.82
CHEMBL5074266 Kd = 1900.0 nM 5.72
CHEMBL5076150 Kd = 1900.0 nM 5.72
CHEMBL5089927 Kd = 3800.0 nM 5.42
CHEMBL5090219 Kd = 5900.0 nM 5.23
CHEMBL5081697 Kd = 6700.0 nM 5.17
CHEMBL5092196 Kd = 7100.0 nM 5.15
CHEMBL5090793 Kd = 8000.0 nM 5.10
CHEMBL5076899 Kd = 9300.0 nM 5.03
CHEMBL5079700 Kd = 9500.0 nM 5.02
CHEMBL5081559 Kd = 15000.0 nM 4.82
CHEMBL5078275 Kd = 71000.0 nM 4.15
CHEMBL511201 Activity < 0.1 uM -
CHEMBL5091172 Kd = 145000.0 nM -