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Assay Detail

CHEMBL5034632

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human alpha9alpha10 nAChR expressed in Xenopus laevis oocytes assessed as inhibition of acetylcholine-induced current response by two-electrode voltage-clamp method
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Oocyte
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha9/alpha10 (CHEMBL2109238)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of Methylene Thioacetal-Incorporated α-RgIA Analogues as Potent and Stable Antagonists of the Human α9α10 Nicotinic Acetylcholine Receptor for the Treatment of Neuropathic Pain.
Zheng N, Christensen SB, Dowell C, Purushottam L, Skalicky JJ, McIntosh JM, Chou DH.
J Med Chem (2021) 64 : 9513 -9524
PubMed 34161094 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 8.04 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5086734 1 9.30
CHEMBL5090753 1 9.05
CHEMBL5089881 1 8.82
CHEMBL5073182 1 8.54
CHEMBL5080428 1 8.21
CHEMBL1836968 1 6.29
CHEMBL5091401 1 6.05
CHEMBL5090616 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5086734 IC50 = 0.5 nM 9.30
CHEMBL5090753 IC50 = 0.9 nM 9.05
CHEMBL5089881 IC50 = 1.5 nM 8.82
CHEMBL5073182 IC50 = 2.9 nM 8.54
CHEMBL5080428 IC50 = 6.1 nM 8.21
CHEMBL1836968 IC50 = 510.0 nM 6.29
CHEMBL5091401 IC50 = 882.0 nM 6.05
CHEMBL5090616 IC50 > 1000.0 nM -