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Assay Detail

CHEMBL5035391

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at KOR (unknown origin) expressed in HEK293-A cells assessed as inhibition of forskolin-induced cAMP accumulation preincubated for 30 mins in presence of 3-isobutyl-1-methylxanthine followed by forskolin and compound addition by competitive PKA binding assay
20
Total Activities
11
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293-A
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kappa-type opioid receptor (CHEMBL237)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of Multifunctional and Orally Active Cyclic Peptide Agonists of Opioid/Neuropeptide FF Receptors that Produce Potent, Long-Lasting, and Peripherally Restricted Antinociception with Diminished Side Effects.
Zhang M, Xu B, Li N, Zhang R, Zhang Q, Shi X, Xu K, Xiao J, Chen D, Niu J, Shi Y, Fang Q.
J Med Chem (2021) 64 : 13394 -13409
PubMed 34465090 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 7.11 7.93
logEC50 2 - -
pEC50 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL440765 U-69593 2 7.93
CHEMBL5085455 2 7.41
CHEMBL5091560 2 7.15
CHEMBL5090298 2 7.09
CHEMBL5082892 2 6.87
CHEMBL5094675 2 6.86
CHEMBL5086601 2 6.50
CHEMBL31421 2 -
CHEMBL70 MORPHINE 4.0 2 -
CHEMBL5085013 1 -
CHEMBL5086463 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL440765 U-69593 EC50 = 11.75 nM 7.93
CHEMBL440765 U-69593 EC50 = 11.9 nM 7.92
CHEMBL5085455 EC50 = 39.0 nM 7.41
CHEMBL5085455 EC50 = 38.9 nM 7.41
CHEMBL5091560 EC50 = 71.2 nM 7.15
CHEMBL5091560 EC50 = 70.79 nM 7.15
CHEMBL5090298 EC50 = 81.3 nM 7.09
CHEMBL5090298 EC50 = 81.28 nM 7.09
CHEMBL5082892 EC50 = 136.0 nM 6.87
CHEMBL5082892 EC50 = 138.04 nM 6.86
CHEMBL5094675 EC50 = 138.04 nM 6.86
CHEMBL5094675 EC50 = 139.0 nM 6.86
CHEMBL5086601 EC50 = 320.0 nM 6.50
CHEMBL5086601 EC50 = 316.23 nM 6.50
CHEMBL5086463 logEC50 - -
CHEMBL5085013 logEC50 - -
CHEMBL70 MORPHINE EC50 - -
CHEMBL31421 EC50 - -
CHEMBL31421 pEC50 - -
CHEMBL70 MORPHINE pEC50 - -