Assay Detail
Binding
CHEMBL5036847
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Activation of wild-type human STING expressed in human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 7 hrs by luciferase reporter gene assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | H69AR |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Stimulator of interferon genes protein (CHEMBL4523377) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Synthesis and Biological Evaluation of Phosphoester and Phosphorothioate Prodrugs of STING Agonist 3',3'-c-Di(2'F,2'dAMP).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 21 | 6.75 | 7.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5079224 | — | — | 1 | 7.70 |
| CHEMBL5075359 | — | — | 1 | 7.70 |
| CHEMBL5074770 | — | — | 1 | 7.70 |
| CHEMBL5079226 | — | — | 1 | 7.52 |
| CHEMBL5073386 | — | — | 1 | 7.52 |
| CHEMBL5089205 | — | — | 1 | 7.40 |
| CHEMBL5076372 | — | — | 1 | 7.30 |
| CHEMBL5078789 | — | — | 1 | 7.30 |
| CHEMBL5080355 | — | — | 1 | 7.30 |
| CHEMBL5093147 | — | — | 1 | 6.96 |
| CHEMBL5089851 | — | — | 1 | 6.82 |
| CHEMBL5093084 | — | — | 1 | 6.75 |
| CHEMBL5080867 | — | — | 1 | 6.70 |
| CHEMBL5083793 | — | — | 1 | 6.57 |
| CHEMBL5090007 | — | — | 1 | 6.51 |
| CHEMBL5076132 | — | — | 1 | 6.44 |
| CHEMBL5093813 | — | — | 1 | 5.99 |
| CHEMBL4435159 | — | — | 1 | 5.58 |
| CHEMBL4577528 | — | — | 1 | 5.57 |
| CHEMBL4449584 | — | — | 1 | 5.32 |
| CHEMBL5082153 | — | — | 1 | 5.11 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5079224 | — | EC50 | = | 20.0 | nM | 7.70 |
| CHEMBL5075359 | — | EC50 | = | 20.0 | nM | 7.70 |
| CHEMBL5074770 | — | EC50 | = | 20.0 | nM | 7.70 |
| CHEMBL5079226 | — | EC50 | = | 30.0 | nM | 7.52 |
| CHEMBL5073386 | — | EC50 | = | 30.0 | nM | 7.52 |
| CHEMBL5089205 | — | EC50 | = | 40.0 | nM | 7.40 |
| CHEMBL5076372 | — | EC50 | = | 50.0 | nM | 7.30 |
| CHEMBL5078789 | — | EC50 | = | 50.0 | nM | 7.30 |
| CHEMBL5080355 | — | EC50 | = | 50.0 | nM | 7.30 |
| CHEMBL5093147 | — | EC50 | = | 110.0 | nM | 6.96 |
| CHEMBL5089851 | — | EC50 | = | 150.0 | nM | 6.82 |
| CHEMBL5093084 | — | EC50 | = | 180.0 | nM | 6.75 |
| CHEMBL5080867 | — | EC50 | = | 200.0 | nM | 6.70 |
| CHEMBL5083793 | — | EC50 | = | 270.0 | nM | 6.57 |
| CHEMBL5090007 | — | EC50 | = | 310.0 | nM | 6.51 |
| CHEMBL5076132 | — | EC50 | = | 360.0 | nM | 6.44 |
| CHEMBL5093813 | — | EC50 | = | 1030.0 | nM | 5.99 |
| CHEMBL4435159 | — | EC50 | = | 2630.0 | nM | 5.58 |
| CHEMBL4577528 | — | EC50 | = | 2710.0 | nM | 5.57 |
| CHEMBL4449584 | — | EC50 | = | 4740.0 | nM | 5.32 |
| CHEMBL5082153 | — | EC50 | = | 7790.0 | nM | 5.11 |