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Assay Detail

CHEMBL5037521

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human aromatase assessed as reduction in fluorescence intensity preincubated with NADPH regenerating system for 10 mins followed by substrate addition incubated for 60 mins by fluorescence based microplate reader analysis
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aromatase (CHEMBL1978)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Balanced dual acting compounds targeting aromatase and estrogen receptor α as an emerging therapeutic opportunity to counteract estrogen responsive breast cancer.
Caciolla J, Martini S, Spinello A, Pavlin M, Turrini E, Simonelli F, Belluti F, Rampa A, Bisi A, Fimognari C, Zaffaroni N, Gobbi S, Magistrato A.
Eur J Med Chem (2021) 224 : 113733 -113733
PubMed 34364162 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 6.64 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1444 LETROZOLE 4.0 1 8.40
CHEMBL5080066 1 7.64
CHEMBL5072101 1 7.46
CHEMBL5074047 1 7.28
CHEMBL5081446 1 7.20
CHEMBL5082681 1 6.22
CHEMBL5069811 1 6.07
CHEMBL5092560 1 6.05
CHEMBL5075144 1 6.02
CHEMBL5087363 1 6.00
CHEMBL5082245 1 5.90
CHEMBL5092843 1 5.49
CHEMBL5077976 1 -
CHEMBL1093458 ENDOXIFEN 3.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1444 LETROZOLE IC50 = 4.0 nM 8.40
CHEMBL5080066 IC50 = 23.0 nM 7.64
CHEMBL5072101 IC50 = 35.0 nM 7.46
CHEMBL5074047 IC50 = 52.0 nM 7.28
CHEMBL5081446 IC50 = 63.0 nM 7.20
CHEMBL5082681 IC50 = 596.0 nM 6.22
CHEMBL5069811 IC50 = 861.0 nM 6.07
CHEMBL5092560 IC50 = 900.0 nM 6.05
CHEMBL5075144 IC50 = 952.0 nM 6.02
CHEMBL5087363 IC50 = 1002.0 nM 6.00
CHEMBL5082245 IC50 = 1253.0 nM 5.90
CHEMBL5092843 IC50 = 3240.0 nM 5.49
CHEMBL5077976 IC50 > 10000.0 nM -
CHEMBL1093458 ENDOXIFEN IC50 > 10000.0 nM -