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Assay Detail

CHEMBL5040063

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of LPS-induced NF-kappaB activation in human THP-1 monocytes expressing luciferase gene pretreated for 30 mins followed by LPS stimulation and measured after 24 hrs by bioluminescence analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type THP-1
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Nuclear factor NF-kappa-B complex (CHEMBL2094258)
Type PROTEIN COMPLEX GROUP
Organism Homo sapiens

Publication

Asymmetric Synthesis and Biological Screening of Quinoxaline-Containing Synthetic Lipoxin A<sub>4</sub> Mimetics (QNX-sLXms).
de Gaetano M, Tighe C, Gahan K, Zanetti A, Chen J, Newson J, Cacace A, Marai M, Gaffney A, Brennan E, Kantharidis P, Cooper ME, Leroy X, Perretti M, Gilroy D, Godson C, Guiry PJ.
J Med Chem (2021) 64 : 9193 -9216
PubMed 34138563 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 10.41 10.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5080827 1 10.60
CHEMBL392438 LIPOXIN A4 1 10.22
CHEMBL5091584 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5080827 IC50 = 0.025 nM 10.60
CHEMBL392438 LIPOXIN A4 IC50 = 0.06 nM 10.22
CHEMBL5091584 IC50 = 0.001 nM -