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Assay Detail

CHEMBL5040285

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SARS-COV2 S-RBD binding to human ACE2 expressed in HEK293T cells using FMZ as substrate by NanoLuc luciferase assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Angiotensin-converting enzyme 2 (CHEMBL3736)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthetic Peptides That Antagonize the Angiotensin-Converting Enzyme-2 (ACE-2) Interaction with SARS-CoV-2 Receptor Binding Spike Protein.
Sadremomtaz A, Al-Dahmani ZM, Ruiz-Moreno AJ, Monti A, Wang C, Azad T, Bell JC, Doti N, Velasco-Velázquez MA, de Jong D, de Jonge J, Smit J, Dömling A, van Goor H, Groves MR.
J Med Chem (2022) 65 : 2836 -2847
PubMed 34328726 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 7.91 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5091821 1 8.22
CHEMBL5088125 1 8.05
CHEMBL5093610 1 8.00
CHEMBL5081268 1 7.96
CHEMBL5081573 1 7.75
CHEMBL5078837 1 7.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5091821 IC50 = 6.0 nM 8.22
CHEMBL5088125 IC50 = 9.0 nM 8.05
CHEMBL5093610 IC50 = 10.0 nM 8.00
CHEMBL5081268 IC50 = 11.0 nM 7.96
CHEMBL5081573 IC50 = 18.0 nM 7.75
CHEMBL5078837 IC50 = 32.0 nM 7.50