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Assay Detail

CHEMBL5041176

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of wild type human P-gp in human MES-SA/Dx5 cells incubated for 3 days by MTT assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MES-SA/Dx5
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ATP-dependent translocase ABCB1 (CHEMBL4302)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and Characterization of Potent Dual P-Glycoprotein and CYP3A4 Inhibitors: Design, Synthesis, Cryo-EM Analysis, and Biological Evaluations.
Urgaonkar S, Nosol K, Said AM, Nasief NN, Bu Y, Locher KP, Lau JYN, Smolinski MP.
J Med Chem (2022) 65 : 191 -216
PubMed 34928144 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 7.16 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5074763 1 8.10
CHEMBL4594298 ENCEQUIDAR 3.0 1 7.96
CHEMBL5076722 1 7.85
CHEMBL5076816 1 7.75
CHEMBL5075886 1 7.46
CHEMBL5081940 1 7.41
CHEMBL5081545 1 7.41
CHEMBL5083331 1 7.39
CHEMBL5077462 1 7.31
CHEMBL5075114 1 7.30
CHEMBL5090486 1 7.21
CHEMBL5076237 1 7.04
CHEMBL5094677 1 7.03
CHEMBL5072078 1 6.68
CHEMBL5073106 1 6.65
CHEMBL5079066 1 6.30
CHEMBL5074563 1 6.00
CHEMBL157101 KETOCONAZOLE 4.0 1 5.98
CHEMBL5077301 1 -
CHEMBL5074836 1 -
CHEMBL5084749 1 -
CHEMBL5080434 1 -
CHEMBL5094781 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5074763 IC50 = 8.0 nM 8.10
CHEMBL4594298 ENCEQUIDAR IC50 = 11.0 nM 7.96
CHEMBL5076722 IC50 = 14.0 nM 7.85
CHEMBL5076816 IC50 = 18.0 nM 7.75
CHEMBL5075886 IC50 = 35.0 nM 7.46
CHEMBL5081940 IC50 = 39.0 nM 7.41
CHEMBL5081545 IC50 = 39.0 nM 7.41
CHEMBL5083331 IC50 = 41.0 nM 7.39
CHEMBL5077462 IC50 = 49.0 nM 7.31
CHEMBL5075114 IC50 = 50.0 nM 7.30
CHEMBL5090486 IC50 = 61.0 nM 7.21
CHEMBL5076237 IC50 = 91.0 nM 7.04
CHEMBL5094677 IC50 = 94.0 nM 7.03
CHEMBL5072078 IC50 = 210.0 nM 6.68
CHEMBL5073106 IC50 = 222.0 nM 6.65
CHEMBL5079066 IC50 = 495.0 nM 6.30
CHEMBL5074563 IC50 = 993.0 nM 6.00
CHEMBL157101 KETOCONAZOLE IC50 = 1058.0 nM 5.98
CHEMBL5077301 IC50 > 1280.0 nM -
CHEMBL5074836 IC50 > 1280.0 nM -
CHEMBL5084749 IC50 > 1280.0 nM -
CHEMBL5080434 IC50 > 1280.0 nM -
CHEMBL5094781 IC50 > 1280.0 nM -