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Assay Detail

CHEMBL5041209

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human Nav1.4 stably expressed in cells with train pulses to -20 mV at 0.1 Hz and holding potential of -95 mV by whole-cell Qube-automated patch clamp assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium channel protein type 4 subunit alpha (CHEMBL2072)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of ProTx-II Analogues as Highly Selective Peptide Blockers of Na<sub>v</sub>1.7 for the Treatment of Pain.
Adams GL, Pall PS, Grauer SM, Zhou X, Ballard JE, Vavrek M, Kraus RL, Morissette P, Li N, Colarusso S, Bianchi E, Palani A, Klein R, John CT, Wang D, Tudor M, Nolting AF, Biba M, Nowak T, Makarov AA, Reibarkh M, Buevich AV, Zhong W, Regalado EL, Wang X, Gao Q, Shahripour A, Zhu Y, de Simone D, Frattarelli T, Pasquini NM, Magotti P, Iaccarino R, Li Y, Solly K, Lee KJ, Wang W, Chen F, Zeng H, Wang J, Regan H, Amin RP, Regan CP, Burgey CS, Henze DA, Sun C, Tellers DM.
J Med Chem (2022) 65 : 485 -496
PubMed 34931831 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 5.70 7.03

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3318147 1 7.03
CHEMBL5074400 1 5.91
CHEMBL5085021 1 5.75
CHEMBL5086465 1 5.38
CHEMBL5088003 1 5.32
CHEMBL5086606 1 4.82
CHEMBL5074736 1 -
CHEMBL5080941 1 -
CHEMBL5091568 1 -
CHEMBL5083009 1 -
CHEMBL5075309 1 -
CHEMBL5085091 1 -
CHEMBL5076349 1 -
CHEMBL5075738 1 -
CHEMBL5092367 1 -
CHEMBL5076456 1 -
CHEMBL5078519 1 -
CHEMBL5093139 1 -
CHEMBL5089216 1 -
CHEMBL5088224 1 -
CHEMBL5078424 1 -
CHEMBL5093042 1 -
CHEMBL5092073 1 -
CHEMBL5090054 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3318147 IC50 = 93.0 nM 7.03
CHEMBL5074400 IC50 = 1242.0 nM 5.91
CHEMBL5085021 IC50 = 1765.0 nM 5.75
CHEMBL5086465 IC50 = 4136.0 nM 5.38
CHEMBL5088003 IC50 = 4774.0 nM 5.32
CHEMBL5086606 IC50 = 15210.0 nM 4.82
CHEMBL5074736 IC50 > 17000.0 nM -
CHEMBL5080941 IC50 > 17000.0 nM -
CHEMBL5091568 IC50 > 17000.0 nM -
CHEMBL5083009 IC50 > 17000.0 nM -
CHEMBL5075309 IC50 > 17000.0 nM -
CHEMBL5085091 IC50 > 17000.0 nM -
CHEMBL5076349 IC50 > 17000.0 nM -
CHEMBL5075738 IC50 > 17000.0 nM -
CHEMBL5092367 IC50 > 17000.0 nM -
CHEMBL5076456 IC50 > 17000.0 nM -
CHEMBL5078519 IC50 > 17000.0 nM -
CHEMBL5093139 IC50 > 17000.0 nM -
CHEMBL5089216 IC50 > 17000.0 nM -
CHEMBL5088224 IC50 > 17000.0 nM -
CHEMBL5078424 IC50 > 17000.0 nM -
CHEMBL5093042 IC50 > 17000.0 nM -
CHEMBL5092073 IC50 > 17000.0 nM -
CHEMBL5090054 IC50 > 17000.0 nM -