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Assay Detail

CHEMBL5043595

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human uPA H99Y mutant using pyroGlu-Gly-Arg-pNA as substrate by absorbance based analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Disruption of Water Networks is the Cause of Human/Mouse Species Selectivity in Urokinase Plasminogen Activator (uPA) Inhibitors Derived from Hexamethylene Amiloride (HMA).
S El Salamouni N, Buckley BJ, Jiang L, Huang M, Ranson M, Kelso MJ, Yu H.
J Med Chem (2022) 65 : 1933 -1945
PubMed 34898192 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 5.16 5.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL945 AMILORIDE 4.0 1 5.23
CHEMBL4206764 1 5.16
CHEMBL4213248 1 5.10
CHEMBL501701 N,N-HEXAMETHYLENEAMILORIDE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL945 AMILORIDE Ki = 5900.0 nM 5.23
CHEMBL4206764 Ki = 6880.0 nM 5.16
CHEMBL4213248 Ki = 8015.0 nM 5.10
CHEMBL501701 N,N-HEXAMETHYLENEAMILORIDE Ki > 50000.0 nM -