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Assay Detail

CHEMBL5043709

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at N-terminal tagged human CB1 receptor transfected with HEK293 cells assessed as forskolin-stimulated cAMP level in presence of CP55940 by BRET assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cannabinoid receptor 1 (CHEMBL218)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of 3-(4-Chlorophenyl)-1-(phenethyl)urea Analogues as Allosteric Modulators of the Cannabinoid Type-1 Receptor: RTICBM-189 is Brain Penetrant and Attenuates Reinstatement of Cocaine-Seeking Behavior.
Nguyen T, Gamage TF, Finlay DB, Decker AM, Langston TL, Barrus D, Glass M, Li JX, Kenakin TP, Zhang Y.
J Med Chem (2022) 65 : 257 -270
PubMed 34929081 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.66 6.17

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5092369 1 6.17
CHEMBL5086597 1 6.14
CHEMBL5093337 1 6.06
CHEMBL5079805 1 6.04
CHEMBL5088172 1 5.94
CHEMBL5080578 1 5.87
CHEMBL5080045 1 5.82
CHEMBL5077926 1 5.77
CHEMBL5087480 1 5.75
CHEMBL5084600 1 5.69
CHEMBL5087483 1 5.49
CHEMBL1360165 1 5.46
CHEMBL1289260 1 5.38
CHEMBL5077368 1 5.36
CHEMBL5081586 1 5.18
CHEMBL5082643 1 5.11
CHEMBL5089573 1 4.97
CHEMBL2299364 1 -
CHEMBL5087934 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5092369 IC50 = 676.08 nM 6.17
CHEMBL5086597 IC50 = 724.44 nM 6.14
CHEMBL5093337 IC50 = 870.96 nM 6.06
CHEMBL5079805 IC50 = 912.01 nM 6.04
CHEMBL5088172 IC50 = 1148.15 nM 5.94
CHEMBL5080578 IC50 = 1348.96 nM 5.87
CHEMBL5080045 IC50 = 1513.56 nM 5.82
CHEMBL5077926 IC50 = 1698.24 nM 5.77
CHEMBL5087480 IC50 = 1778.28 nM 5.75
CHEMBL5084600 IC50 = 2041.74 nM 5.69
CHEMBL5087483 IC50 = 3235.94 nM 5.49
CHEMBL1360165 IC50 = 3467.37 nM 5.46
CHEMBL1289260 IC50 = 4168.69 nM 5.38
CHEMBL5077368 IC50 = 4365.16 nM 5.36
CHEMBL5081586 IC50 = 6606.93 nM 5.18
CHEMBL5082643 IC50 = 7762.47 nM 5.11
CHEMBL5089573 IC50 = 10715.19 nM 4.97
CHEMBL2299364 IC50 > 10000.0 nM -
CHEMBL5087934 IC50 > 10000.0 nM -