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Assay Detail

CHEMBL5044069

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human APC-labeled RORgammat LBD (262 to 518 residues) transfected in Escherichia coli BL21 (DE3) assessed as biotinylated SRC recruitment incubated for 1 hr by dual FRET assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nuclear receptor ROR-gamma (CHEMBL1741186)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Orally Available Retinoic Acid Receptor-Related Orphan Receptor γ-t/Dihydroorotate Dehydrogenase Dual Inhibitors for the Treatment of Refractory Inflammatory Bowel Disease.
Chen JA, Ma H, Liu Z, Tian J, Lu S, Fang W, Ze S, Lu W, Xie Q, Huang J, Wang Y.
J Med Chem (2022) 65 : 592 -615
PubMed 34957834 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.92 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3609408 1 8.00
CHEMBL5075214 1 6.83
CHEMBL5093830 1 6.68
CHEMBL62136 T091317 1 6.62
CHEMBL5086318 1 6.49
CHEMBL5075277 1 -
CHEMBL5082345 1 -
CHEMBL5086624 1 -
CHEMBL5085951 1 -
CHEMBL5089276 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3609408 IC50 = 10.0 nM 8.00
CHEMBL5075214 IC50 = 149.0 nM 6.83
CHEMBL5093830 IC50 = 211.0 nM 6.68
CHEMBL62136 T091317 IC50 = 238.0 nM 6.62
CHEMBL5086318 IC50 = 325.0 nM 6.49
CHEMBL5075277 IC50 - -
CHEMBL5082345 IC50 - -
CHEMBL5086624 IC50 - -
CHEMBL5085951 IC50 - -
CHEMBL5089276 IC50 - -