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Assay Detail

CHEMBL5044432

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity BRD3 BD2 (unknown origin) incubated for 1 hr by TR-FRET assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 3 (CHEMBL1795186)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery, X-ray Crystallography, and Anti-inflammatory Activity of Bromodomain-containing Protein 4 (BRD4) BD1 Inhibitors Targeting a Distinct New Binding Site.
Liu Z, Li Y, Chen H, Lai HT, Wang P, Wu SY, Wold EA, Leonard PG, Joseph S, Hu H, Chiang CM, Brasier AR, Tian B, Zhou J.
J Med Chem (2022) 65 : 2388 -2408
PubMed 34982556 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.30 7.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1957266 1 7.14
CHEMBL2393130 APABETALONE 3.0 1 6.66
CHEMBL5090401 1 5.70
CHEMBL5076336 1 5.70
CHEMBL5080616 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1957266 IC50 = 72.0 nM 7.14
CHEMBL2393130 APABETALONE IC50 = 217.0 nM 6.66
CHEMBL5090401 IC50 = 1996.0 nM 5.70
CHEMBL5076336 IC50 = 2018.0 nM 5.70
CHEMBL5080616 IC50 - -