Assay Detail
Binding
CHEMBL5044664
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of full length recombinant human C-terminal His tagged HDAC8 expressed in baculovirus infected Sf9 insect cells using Ac-Leu-GlyLys (Tfa)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence microtiter plate reader assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Discovery of Novel Histone Deacetylase 6 (HDAC6) Inhibitors with Enhanced Antitumor Immunity of Anti-PD-L1 Immunotherapy in Melanoma.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 14 | 5.15 | 5.88 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL98 | VORINOSTAT | 4.0 | 1 | 5.88 |
| CHEMBL5079482 | — | — | 1 | 5.65 |
| CHEMBL5069541 | — | — | 1 | 5.59 |
| CHEMBL5076163 | — | — | 1 | 5.43 |
| CHEMBL5093602 | — | — | 1 | 5.28 |
| CHEMBL511749 | — | — | 1 | 5.21 |
| CHEMBL5093832 | — | — | 1 | 5.04 |
| CHEMBL5082644 | — | — | 1 | 4.99 |
| CHEMBL5077674 | — | — | 1 | 4.94 |
| CHEMBL5079646 | — | — | 1 | 4.87 |
| CHEMBL5070026 | — | — | 1 | 4.85 |
| CHEMBL5092352 | — | — | 1 | 4.84 |
| CHEMBL5080669 | — | — | 1 | 4.82 |
| CHEMBL5085871 | — | — | 1 | 4.70 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL98 | VORINOSTAT | IC50 | = | 1320.0 | nM | 5.88 |
| CHEMBL5079482 | — | IC50 | = | 2220.0 | nM | 5.65 |
| CHEMBL5069541 | — | IC50 | = | 2560.0 | nM | 5.59 |
| CHEMBL5076163 | — | IC50 | = | 3740.0 | nM | 5.43 |
| CHEMBL5093602 | — | IC50 | = | 5220.0 | nM | 5.28 |
| CHEMBL511749 | — | IC50 | = | 6210.0 | nM | 5.21 |
| CHEMBL5093832 | — | IC50 | = | 9020.0 | nM | 5.04 |
| CHEMBL5082644 | — | IC50 | = | 10190.0 | nM | 4.99 |
| CHEMBL5077674 | — | IC50 | = | 11550.0 | nM | 4.94 |
| CHEMBL5079646 | — | IC50 | = | 13370.0 | nM | 4.87 |
| CHEMBL5070026 | — | IC50 | = | 14180.0 | nM | 4.85 |
| CHEMBL5092352 | — | IC50 | = | 14560.0 | nM | 4.84 |
| CHEMBL5080669 | — | IC50 | = | 15230.0 | nM | 4.82 |
| CHEMBL5085871 | — | IC50 | = | 19810.0 | nM | 4.70 |