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Assay Detail

CHEMBL5045401

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Time dependent inhibition of CYP3A in human liver microsomes incubated for 30 mins in presence of NADPH generating system by LC-MS/MS analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Tissue Liver
Subcellular Microsomes
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Unique Oxidative Metabolism of Bufalin Generates Two Reactive Metabolites That Strongly Inactivate Human Cytochrome P450 3A.
Tu D, Ning J, Zou L, Wang P, Zhang Y, Tian X, Zhang F, Zheng J, Ge G.
J Med Chem (2022) 65 : 4018 -4029
PubMed 35094507 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.37 6.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2068968 1 6.43
CHEMBL451719 1 5.23
CHEMBL399680 BUFALIN 1 4.94
CHEMBL2069016 1 4.87

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2068968 IC50 = 370.0 nM 6.43
CHEMBL451719 IC50 = 5930.0 nM 5.23
CHEMBL399680 BUFALIN IC50 = 11420.0 nM 4.94
CHEMBL2069016 IC50 = 13400.0 nM 4.87