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Assay Detail

CHEMBL5046688

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human P2X7R expressed in HEK293 cells assessed as reduction in YO-PRO-1 iodide dye uptake preincubated for 30 mins followed by YO-PRO-1 iodide addition and measured after 2 hrs by fluorescence plate reader analysis
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 7 (CHEMBL4805)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Piperazine squaric acid diamides, a novel class of allosteric P2X7 receptor antagonists.
Patberg M, Isaak A, Füsser F, Ortiz Zacarías NV, Vinnenberg L, Schulte J, Michetti L, Grey L, van der Horst C, Hundehege P, Koch O, Heitman LH, Budde T, Junker A.
Eur J Med Chem (2021) 226 : 113838 -113838
PubMed 34571173 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 6.40 7.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2338352 1 7.80
CHEMBL5079389 1 7.62
CHEMBL5093653 1 7.19
CHEMBL5089768 1 7.13
CHEMBL5094161 1 7.06
CHEMBL5075280 1 6.99
CHEMBL5072113 1 6.96
CHEMBL5076450 1 6.87
CHEMBL5093681 1 6.82
CHEMBL5079624 1 6.80
CHEMBL5094084 1 6.65
CHEMBL5092737 1 6.52
CHEMBL5073035 1 6.51
CHEMBL5081860 1 6.42
CHEMBL5076226 1 6.31
CHEMBL5082947 1 6.26
CHEMBL5090437 1 6.13
CHEMBL5084591 1 6.12
CHEMBL5075398 1 6.00
CHEMBL5074653 1 4.31
CHEMBL5092738 1 4.31
CHEMBL5088972 1 4.08

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2338352 IC50 = 15.85 nM 7.80
CHEMBL5079389 IC50 = 23.99 nM 7.62
CHEMBL5093653 IC50 = 64.57 nM 7.19
CHEMBL5089768 IC50 = 74.13 nM 7.13
CHEMBL5094161 IC50 = 87.1 nM 7.06
CHEMBL5075280 IC50 = 102.33 nM 6.99
CHEMBL5072113 IC50 = 109.65 nM 6.96
CHEMBL5076450 IC50 = 134.9 nM 6.87
CHEMBL5093681 IC50 = 151.36 nM 6.82
CHEMBL5079624 IC50 = 158.49 nM 6.80
CHEMBL5094084 IC50 = 223.87 nM 6.65
CHEMBL5092737 IC50 = 302.0 nM 6.52
CHEMBL5073035 IC50 = 309.03 nM 6.51
CHEMBL5081860 IC50 = 380.19 nM 6.42
CHEMBL5076226 IC50 = 489.78 nM 6.31
CHEMBL5082947 IC50 = 549.54 nM 6.26
CHEMBL5090437 IC50 = 741.31 nM 6.13
CHEMBL5084591 IC50 = 758.58 nM 6.12
CHEMBL5075398 IC50 = 1000.0 nM 6.00
CHEMBL5074653 IC50 = 48977.88 nM 4.31
CHEMBL5092738 IC50 = 48977.88 nM 4.31
CHEMBL5088972 IC50 = 83176.38 nM 4.08