Assay Detail
Binding
CHEMBL5047046
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Inhibition of hedgehog signalling in mouse ASZ001 cells assessed as reduction in Gli1 mRNA expression measured after 48 hrs by RT-PCR analysis
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | ASZ001 |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Affinity-based protein profiling identifies vitamin D3 as a heat shock protein 70 antagonist that regulates hedgehog transduction in murine basal cell carcinoma.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 14 | 6.08 | 7.05 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL846 | CALCITRIOL | 4.0 | 1 | 7.05 |
| CHEMBL5078522 | — | — | 1 | 6.58 |
| CHEMBL5092119 | — | — | 1 | 6.42 |
| CHEMBL1040 | CALCIFEDIOL ANHYDROUS | 4.0 | 1 | 6.40 |
| CHEMBL5081148 | — | — | 1 | 6.38 |
| CHEMBL5091673 | — | — | 1 | 6.37 |
| CHEMBL3416503 | — | — | 1 | 6.24 |
| CHEMBL5084820 | — | — | 1 | 6.15 |
| CHEMBL5076625 | — | — | 1 | 5.96 |
| CHEMBL5085638 | — | — | 1 | 5.77 |
| CHEMBL33859 | — | — | 1 | 5.75 |
| CHEMBL1042 | CHOLECALCIFEROL | 4.0 | 1 | 5.68 |
| CHEMBL5073842 | — | — | 1 | 5.47 |
| CHEMBL5086005 | — | — | 1 | 4.86 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL846 | CALCITRIOL | IC50 | = | 89.0 | nM | 7.05 |
| CHEMBL5078522 | — | IC50 | = | 260.0 | nM | 6.58 |
| CHEMBL5092119 | — | IC50 | = | 380.0 | nM | 6.42 |
| CHEMBL1040 | CALCIFEDIOL ANHYDROUS | IC50 | = | 400.0 | nM | 6.40 |
| CHEMBL5081148 | — | IC50 | = | 420.0 | nM | 6.38 |
| CHEMBL5091673 | — | IC50 | = | 430.0 | nM | 6.37 |
| CHEMBL3416503 | — | IC50 | = | 580.0 | nM | 6.24 |
| CHEMBL5084820 | — | IC50 | = | 710.0 | nM | 6.15 |
| CHEMBL5076625 | — | IC50 | = | 1100.0 | nM | 5.96 |
| CHEMBL5085638 | — | IC50 | = | 1700.0 | nM | 5.77 |
| CHEMBL33859 | — | IC50 | = | 1800.0 | nM | 5.75 |
| CHEMBL1042 | CHOLECALCIFEROL | IC50 | = | 2100.0 | nM | 5.68 |
| CHEMBL5073842 | — | IC50 | = | 3400.0 | nM | 5.47 |
| CHEMBL5086005 | — | IC50 | = | 13800.0 | nM | 4.86 |