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Assay Detail

CHEMBL5048418

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-3C fused human RET (705 to 1013 residues) expressed in sf9 baculovirus expression system using 5-FAM- peptide as substrate preincubated for 1 hr followed by substrate addition in presence of ATP by microfluidics assay
36
Total Activities
36
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proto-oncogene tyrosine-protein kinase receptor Ret (CHEMBL2041)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of N-Trisubstituted Pyrimidine Derivatives as Type I RET and RET Gatekeeper Mutant Inhibitors with a Novel Kinase Binding Pose.
Zhang L, Moccia M, Briggs DC, Bharate JB, Lakkaniga NR, Knowles P, Yan W, Tran P, Kharbanda A, Wang X, Leung YK, Frett B, Santoro M, McDonald NQ, Carlomagno F, Li HY.
J Med Chem (2022) 65 : 1536 -1551
PubMed 35081714 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 36 7.47 8.91

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4559134 SELPERCATINIB 4.0 1 8.91
CHEMBL5087283 1 8.29
CHEMBL5074882 1 8.26
CHEMBL5080296 1 8.23
CHEMBL5073804 1 8.22
CHEMBL5070593 1 8.21
CHEMBL5078090 1 8.19
CHEMBL5094848 1 8.14
CHEMBL5089911 1 8.12
CHEMBL5093057 1 8.08
CHEMBL5085579 1 7.98
CHEMBL5094196 1 7.91
CHEMBL5089027 1 7.88
CHEMBL5086966 1 7.87
CHEMBL5079216 1 7.80
CHEMBL5092920 1 7.73
CHEMBL5075257 1 7.66
CHEMBL5087974 1 7.61
CHEMBL5081199 1 7.55
CHEMBL5084023 1 7.38
CHEMBL5078263 1 7.18
CHEMBL5078934 1 7.03
CHEMBL5075735 1 6.99
CHEMBL5081142 1 6.99
CHEMBL5093178 1 6.93
CHEMBL5073056 1 6.86
CHEMBL5083534 1 6.85
CHEMBL5071633 1 6.83
CHEMBL1336 SORAFENIB 4.0 1 6.82
CHEMBL5083224 1 6.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4559134 SELPERCATINIB IC50 = 1.24 nM 8.91
CHEMBL5087283 IC50 = 5.07 nM 8.29
CHEMBL5074882 IC50 = 5.45 nM 8.26
CHEMBL5080296 IC50 = 5.82 nM 8.23
CHEMBL5073804 IC50 = 6.01 nM 8.22
CHEMBL5070593 IC50 = 6.2 nM 8.21
CHEMBL5078090 IC50 = 6.43 nM 8.19
CHEMBL5094848 IC50 = 7.27 nM 8.14
CHEMBL5089911 IC50 = 7.53 nM 8.12
CHEMBL5093057 IC50 = 8.31 nM 8.08
CHEMBL5085579 IC50 = 10.52 nM 7.98
CHEMBL5094196 IC50 = 12.41 nM 7.91
CHEMBL5089027 IC50 = 13.09 nM 7.88
CHEMBL5086966 IC50 = 13.45 nM 7.87
CHEMBL5079216 IC50 = 15.87 nM 7.80
CHEMBL5092920 IC50 = 18.77 nM 7.73
CHEMBL5075257 IC50 = 22.1 nM 7.66
CHEMBL5087974 IC50 = 24.7 nM 7.61
CHEMBL5081199 IC50 = 28.11 nM 7.55
CHEMBL5084023 IC50 = 41.29 nM 7.38
CHEMBL5078263 IC50 = 66.58 nM 7.18
CHEMBL5078934 IC50 = 92.97 nM 7.03
CHEMBL5075735 IC50 = 102.51 nM 6.99
CHEMBL5081142 IC50 = 102.14 nM 6.99
CHEMBL5093178 IC50 = 116.1 nM 6.93
CHEMBL5073056 IC50 = 138.0 nM 6.86
CHEMBL5083534 IC50 = 140.65 nM 6.85
CHEMBL5071633 IC50 = 146.6 nM 6.83
CHEMBL1336 SORAFENIB IC50 = 152.3 nM 6.82
CHEMBL5083224 IC50 = 160.13 nM 6.80
CHEMBL5078777 IC50 = 189.57 nM 6.72
CHEMBL5074502 IC50 = 212.3 nM 6.67
CHEMBL5088213 IC50 = 245.95 nM 6.61
CHEMBL5080275 IC50 = 252.25 nM 6.60
CHEMBL5078648 IC50 = 260.15 nM 6.58
CHEMBL5083904 IC50 = 322.4 nM 6.49