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Assay Detail

CHEMBL5048619

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human full-length HDAC2 preincubated for 20 mins with DTT followed by enzyme addition and measured after 10 mins by Glo-luminescence assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 2 (CHEMBL1937)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of a Novel, Small-Molecule Brain-Penetrant Histone Deacetylase Inhibitor That Enhances Spatial Memory Formation in Mice.
Belayet JB, Beamish S, Rahaman M, Alanani S, Virdi RS, Frick DN, Rahman AFMT, Ulicki JS, Biswas S, Arnold LA, Roni MSR, Cheng EY, Steeber DA, Frick KM, Hossain MM.
J Med Chem (2022) 65 : 3388 -3403
PubMed 35133171 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 4.77 5.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5086240 1 5.00
CHEMBL5087342 1 4.96
CHEMBL5081652 1 4.77
CHEMBL5085306 1 4.75
CHEMBL5084611 1 4.60
CHEMBL5074871 1 4.55

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5086240 IC50 = 10000.0 nM 5.00
CHEMBL5087342 IC50 = 11000.0 nM 4.96
CHEMBL5081652 IC50 = 17000.0 nM 4.77
CHEMBL5085306 IC50 = 18000.0 nM 4.75
CHEMBL5084611 IC50 = 25000.0 nM 4.60
CHEMBL5074871 IC50 = 28000.0 nM 4.55