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Assay Detail

CHEMBL5048666

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length N-terminal GST fused human CDK7 (1 to 346 residues)/CyclinH (1 to 323 residues)/ MAT1 (1 to 309 residues) expressed in baculovirus expression system using FL peptide as substrate preincubated for 30 mins followed by substrate addition and further incubated for 90 mins by EZ reader method
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

CDK7/Cyclin H/MNAT1 (CHEMBL3038473)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery and Optimization of Highly Selective Inhibitors of CDK5.
Daniels MH, Malojcic G, Clugston SL, Williams B, Coeffet-Le Gal M, Pan-Zhou XR, Venkatachalan S, Harmange JC, Ledeboer M.
J Med Chem (2022) 65 : 3575 -3596
PubMed 35143203 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 6.68 7.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5080866 1 7.14
CHEMBL5078192 1 7.11
CHEMBL5088480 1 7.06
CHEMBL5077028 1 7.03
CHEMBL5091792 1 7.02
CHEMBL5080361 1 6.98
CHEMBL5090019 1 6.83
CHEMBL5070707 1 6.79
CHEMBL5080566 1 6.77
CHEMBL5088595 1 6.71
CHEMBL5087911 1 6.53
CHEMBL5079342 1 6.48
CHEMBL5083778 1 6.44
CHEMBL5080813 1 6.20
CHEMBL5084200 1 6.14
CHEMBL5084882 1 6.14
CHEMBL5080123 1 6.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5080866 IC50 = 73.0 nM 7.14
CHEMBL5078192 IC50 = 78.0 nM 7.11
CHEMBL5088480 IC50 = 87.0 nM 7.06
CHEMBL5077028 IC50 = 94.0 nM 7.03
CHEMBL5091792 IC50 = 96.0 nM 7.02
CHEMBL5080361 IC50 = 104.0 nM 6.98
CHEMBL5090019 IC50 = 147.0 nM 6.83
CHEMBL5070707 IC50 = 161.0 nM 6.79
CHEMBL5080566 IC50 = 171.0 nM 6.77
CHEMBL5088595 IC50 = 194.0 nM 6.71
CHEMBL5087911 IC50 = 294.0 nM 6.53
CHEMBL5079342 IC50 = 334.0 nM 6.48
CHEMBL5083778 IC50 = 362.0 nM 6.44
CHEMBL5080813 IC50 = 631.0 nM 6.20
CHEMBL5084200 IC50 = 718.0 nM 6.14
CHEMBL5084882 IC50 = 729.0 nM 6.14
CHEMBL5080123 IC50 = 784.0 nM 6.11