Assay Detail
Binding
CHEMBL5053563
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human SHIP2 catalytic domain (419 to 832 residues) phosphatase activity assessed as inhibition of Ins(1,3,4,5)P4 production using Ins(1,3,4,5)P4 as substrate incubated for 20 mins measured using microplate reader
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2 (CHEMBL2331064) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Allosteric Site on SHIP2 Identified Through Fluorescent Ligand Screening and Crystallography: A Potential New Target for Intervention.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 11 | 5.49 | 6.16 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5087243 | — | — | 1 | 6.16 |
| CHEMBL5078323 | — | — | 1 | 6.05 |
| CHEMBL5081948 | — | — | 1 | 5.60 |
| CHEMBL1601846 | — | — | 1 | 5.58 |
| CHEMBL5080660 | — | — | 1 | 5.57 |
| CHEMBL5092991 | — | — | 1 | 5.47 |
| CHEMBL66953 | PURPUROGALLIN | — | 1 | 5.14 |
| CHEMBL1756 | ESTRAMUSTINE PHOSPHATE | 4.0 | 1 | 4.38 |
| CHEMBL23552 | — | — | 1 | - |
| CHEMBL234338 | — | — | 1 | - |
| CHEMBL2337806 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5087243 | — | IC50 | = | 700.0 | nM | 6.16 |
| CHEMBL5078323 | — | IC50 | = | 900.0 | nM | 6.05 |
| CHEMBL5081948 | — | IC50 | = | 2500.0 | nM | 5.60 |
| CHEMBL1601846 | — | IC50 | = | 2600.0 | nM | 5.58 |
| CHEMBL5080660 | — | IC50 | = | 2700.0 | nM | 5.57 |
| CHEMBL5092991 | — | IC50 | = | 3400.0 | nM | 5.47 |
| CHEMBL66953 | PURPUROGALLIN | IC50 | = | 7300.0 | nM | 5.14 |
| CHEMBL1756 | ESTRAMUSTINE PHOSPHATE | IC50 | = | 41700.0 | nM | 4.38 |
| CHEMBL23552 | — | IC50 | - | — | - | |
| CHEMBL234338 | — | IC50 | - | — | - | |
| CHEMBL2337806 | — | IC50 | - | — | - |