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Assay Detail

CHEMBL5053568

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SHIP2 in serum-starved mouse Mm1 cells assessed as reduction in Akt phosphorylation incubated for 30 mins by Western blot analysis
3
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type Mm1
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Allosteric Site on SHIP2 Identified Through Fluorescent Ligand Screening and Crystallography: A Potential New Target for Intervention.
Whitfield H, Hemmings AM, Mills SJ, Baker K, White G, Rushworth S, Riley AM, Potter BVL, Brearley CA.
J Med Chem (2021) 64 : 3813 -3826
PubMed 33724834 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Inhibition 2 - -
IC50 1 6.41 6.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4538474 1 6.41
CHEMBL66953 PURPUROGALLIN 1 -
CHEMBL2337806 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4538474 IC50 = 390.0 nM 6.41
CHEMBL66953 PURPUROGALLIN Inhibition - % -
CHEMBL2337806 Inhibition - % -