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Assay Detail

CHEMBL5054286

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of biotin-tagged MRPEPATIAQELRRIGDEFNA from C-terminal His-tagged human BCL-2 (1` to 211 residues) expressed in Escherichia coli measured after 2 hrs by AlphaScreen assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Apoptosis regulator Bcl-2 (CHEMBL4860)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Novel BCL-X<sub>L</sub> PROTAC Degrader with Enhanced BCL-2 Inhibition.
Pal P, Thummuri D, Lv D, Liu X, Zhang P, Hu W, Poddar SK, Hua N, Khan S, Yuan Y, Zhang X, Zhou D, Zheng G.
J Med Chem (2021) 64 : 14230 -14246
PubMed 34533954 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 4 8.90 9.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL443684 NAVITOCLAX 3.0 1 9.19
CHEMBL5077514 1 8.91
CHEMBL5073297 1 8.83
CHEMBL5087367 1 8.65

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL443684 NAVITOCLAX Kd = 0.65 nM 9.19
CHEMBL5077514 Kd = 1.22 nM 8.91
CHEMBL5073297 Kd = 1.47 nM 8.83
CHEMBL5087367 Kd = 2.25 nM 8.65