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Assay Detail

CHEMBL5055693

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human Nav1.5 expressed in HEK293 cells incubated for 1 hr in presence of veratridine and anthropleurin-C by sodium influx assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium channel protein type 5 subunit alpha (CHEMBL1980)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Acyl-sulfonamide Na<sub>v</sub>1.7 Inhibitors GDC-0276 and GDC-0310.
Safina BS, McKerrall SJ, Sun S, Chen CA, Chowdhury S, Jia Q, Li J, Zenova AY, Andrez JC, Bankar G, Bergeron P, Chang JH, Chang E, Chen J, Dean R, Decker SM, DiPasquale A, Focken T, Hemeon I, Khakh K, Kim A, Kwan R, Lindgren A, Lin S, Maher J, Mezeyova J, Misner D, Nelkenbrecher K, Pang J, Reese R, Shields SD, Sojo L, Sheng T, Verschoof H, Waldbrook M, Wilson MS, Xie Z, Young C, Zabka TS, Hackos DH, Ortwine DF, White AD, Johnson JP, Robinette CL, Dehnhardt CM, Cohen CJ, Sutherlin DP.
J Med Chem (2021) 64 : 2953 -2966
PubMed 33682420 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 5.76 6.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3657939 1 6.96
CHEMBL3657932 1 6.58
CHEMBL5091342 1 6.23
CHEMBL5082739 1 6.12
CHEMBL3657855 1 6.06
CHEMBL3657851 1 5.96
CHEMBL3662226 1 5.96
CHEMBL3657938 1 5.82
CHEMBL5094715 1 5.82
CHEMBL5079176 1 5.75
CHEMBL5082131 1 5.41
CHEMBL5080495 1 5.19
CHEMBL5082321 1 5.16
CHEMBL5071877 1 4.77
CHEMBL5080891 1 4.57
CHEMBL5083410 1 -
CHEMBL5081884 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3657939 IC50 = 110.0 nM 6.96
CHEMBL3657932 IC50 = 260.0 nM 6.58
CHEMBL5091342 IC50 = 590.0 nM 6.23
CHEMBL5082739 IC50 = 750.0 nM 6.12
CHEMBL3657855 IC50 = 870.0 nM 6.06
CHEMBL3657851 IC50 = 1100.0 nM 5.96
CHEMBL3662226 IC50 = 1100.0 nM 5.96
CHEMBL3657938 IC50 = 1500.0 nM 5.82
CHEMBL5094715 IC50 = 1500.0 nM 5.82
CHEMBL5079176 IC50 = 1800.0 nM 5.75
CHEMBL5082131 IC50 = 3900.0 nM 5.41
CHEMBL5080495 IC50 = 6400.0 nM 5.19
CHEMBL5082321 IC50 = 7000.0 nM 5.16
CHEMBL5071877 IC50 = 17000.0 nM 4.77
CHEMBL5080891 IC50 = 27000.0 nM 4.57
CHEMBL5083410 IC50 > 30000.0 nM -
CHEMBL5081884 IC50 > 30000.0 nM -