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Assay Detail

CHEMBL5058461

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Modulation of human 5HT2C expressed in HEK293T cells co-transfected with Galphaq-RLuc8, Ggamma1-GFP2 and Gbeta1 assessed as dissociation of Galphaq from Ggamma1 preincubated for 1 hr followed by addition of coelenterazine 400a substrate and measured after 15 mins by BRET assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2C (CHEMBL225)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Improved 5-HT2 Selective Receptor Modulators for the Treatment of Psychological Disorders.
Kargbo RB.
ACS Med Chem Lett (2021) 12 : 1876 -1878
PubMed 34917242 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 8.41 9.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5078731 1 9.92
CHEMBL5077004 1 9.05
CHEMBL5071818 1 8.57
CHEMBL5085055 1 8.55
CHEMBL65547 PSILOCIN 2.0 1 8.11
CHEMBL197664 1 8.02
CHEMBL5081637 1 7.83
CHEMBL5079070 1 7.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5078731 IC50 = 0.12 nM 9.92
CHEMBL5077004 IC50 = 0.9 nM 9.05
CHEMBL5071818 IC50 = 2.7 nM 8.57
CHEMBL5085055 IC50 = 2.8 nM 8.55
CHEMBL65547 PSILOCIN IC50 = 7.79 nM 8.11
CHEMBL197664 IC50 = 9.51 nM 8.02
CHEMBL5081637 IC50 = 14.9 nM 7.83
CHEMBL5079070 IC50 = 63.5 nM 7.20