Assay Detail
Binding
CHEMBL5059547
Review assay metadata, readout intent, target linkage, and publication context from the same page.
HyPerBlu SMOX enzymatic assay
2
Total Activities
2
Compounds Tested
1
Activity Types
3
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Data for DCP probe JNJ-9350
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 8.00 | 8.00 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| Additional information | Additional information | - | — | In this assay spermine oxidase catalyzes the formation of spermidine, 3 -aminopropanal and hydrogen peroxide using oxygen and spermine as substrates. The addition of Lumigen HyperBlu reagent quenches the reaction and directly reacts with hydrogen peroxide to generate a chemiluminescence signal measured on a BMG PHERAStar FS/FSX multilabel plate reader. |
| Assay publication | Assay publication | - | — | Slide 6, 14-23 in JNJ-9350 Inhibitor for SMOX.pdf |
| Target protein length | Target protein length | - | — | full length human SMOX with N-terminal his tag |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5094181 | — | — | 1 | 8.00 |
| CHEMBL5092747 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5094181 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL5092747 | — | IC50 | > | 31000.0 | nM | - |