Assay Detail
Binding
CHEMBL5059549
Review assay metadata, readout intent, target linkage, and publication context from the same page.
SMOX CETSA assay
1
Total Activities
1
Compounds Tested
1
Activity Types
4
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Data for DCP probe JNJ-9350
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 5.92 | 5.92 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| Additional information | Additional information | - | — | Intact SMOX is detected using alphaLISA. The optimal melting temperature (54°C) of hSMOX for CETSA was used. |
| Assay publication | Assay publication | - | — | Slide 10,11, 38-43 in JNJ-9350 Inhibitor for SMOX.pdf |
| Target protein length | Target protein length | - | — | full length, endogenous human SMOX |
| Target protein type | Target protein type | - | — | A549 cells (adenocarcinomic human alveolar basal epithelial cells that express relatively high levels\nof SMOX) |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5094181 | — | — | 1 | 5.92 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5094181 | — | IC50 | = | 1200.0 | nM | 5.92 |