Assay Detail
Binding
CHEMBL5059550
Review assay metadata, readout intent, target linkage, and publication context from the same page.
HyPerBlu POAX enzymatic assay
2
Total Activities
2
Compounds Tested
1
Activity Types
3
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Peroxisomal N(1)-acetyl-spermine/spermidine oxidase (CHEMBL2105) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Data for DCP probe JNJ-9350
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 5.89 | 6.10 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| Additional information | Additional information | - | — | In this assay Polyamine Oxidase catalyzes the formation of spermidine, 3-acetamidopropanal and hydrogen peroxide using oxygen and N1-acetylspermine as substrates. The addition of 25 µM chlorhexidine compound mixed with the Lumigen HyPerBlu reagent is added to quench the reaction and directly react with hydrogen peroxide, respectively, to generate a chemiluminescence signal measured on the BMG PHERAStar plate reader. |
| Assay publication | Assay publication | - | — | Slide 27-37 in JNJ-9350 Inhibitor for SMOX.pdf |
| Target protein length | Target protein length | - | — | full length human PAOX with N-terminal his tag |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5094181 | — | — | 1 | 6.10 |
| CHEMBL5092747 | — | — | 1 | 5.68 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5094181 | — | IC50 | = | 790.0 | nM | 6.10 |
| CHEMBL5092747 | — | IC50 | = | 2100.0 | nM | 5.68 |