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Assay Detail

CHEMBL5099501

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Anti-necroptotic activity in TSZ induced human HT-29 cells assessed as increase in cell viability pretreated with compound followed by TSZ stimulation and measured after 16 hrs by celltiter-glo luminescent cell viability assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 1 — Organism
Curated By Autocuration

Target

HT-29 (CHEMBL384)
Type CELL-LINE
Organism Homo sapiens

Publication

Discovery of a Trifluoromethoxy Cyclopentanone Benzothiazole Receptor-Interacting Protein Kinase 1 Inhibitor as the Treatment for Alzheimer's Disease.
Sun Y, Xu L, Shao H, Quan D, Mo Z, Wang J, Zhang W, Yu J, Zhuang C, Xu K.
J Med Chem (2022) 65 : 14957 -14969
PubMed 36288088 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 9 7.88 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5172295 1 8.70
CHEMBL4071864 GSK2982772 2.0 1 8.44
CHEMBL5185667 1 8.10
CHEMBL5173571 1 8.00
CHEMBL5189581 1 8.00
CHEMBL5179151 1 7.92
CHEMBL5171828 1 7.89
CHEMBL5189681 1 7.09
CHEMBL5205822 1 6.77

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5172295 EC50 = 2.0 nM 8.70
CHEMBL4071864 GSK2982772 EC50 = 3.6 nM 8.44
CHEMBL5185667 EC50 = 8.0 nM 8.10
CHEMBL5173571 EC50 = 10.0 nM 8.00
CHEMBL5189581 EC50 = 10.0 nM 8.00
CHEMBL5179151 EC50 = 12.0 nM 7.92
CHEMBL5171828 EC50 = 13.0 nM 7.89
CHEMBL5189681 EC50 = 82.0 nM 7.09
CHEMBL5205822 EC50 = 170.0 nM 6.77