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Assay Detail

CHEMBL5099992

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Binding
Binding affinity to recombinant human CaMK2alpha 6x hub domain (345 to 475 residues) expressed in Escherichia coli BL21 DE3 assessed as dissociation constant by surface plasmon resonance assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Exploring the NCS-382 Scaffold for CaMKIIα Modulation: Synthesis, Biochemical Pharmacology, and Biophysical Characterization of Ph-HTBA as a Novel High-Affinity Brain-Penetrant Stabilizer of the CaMKIIα Hub Domain.
Tian Y, Shehata MA, Gauger SJ, Veronesi C, Hamborg L, Thiesen L, Bruus-Jensen J, Royssen JS, Leurs U, Larsen ASG, Krall J, Solbak SMØ, Wellendorph P, Frølund B.
J Med Chem (2022) 65 : 15066 -15084
PubMed 36346645 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 3 5.58 6.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5170093 1 6.12
CHEMBL5170394 1 5.58
CHEMBL472694 1 5.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5170093 Kd = 757.0 nM 6.12
CHEMBL5170394 Kd = 2600.0 nM 5.58
CHEMBL472694 Kd = 8900.0 nM 5.05