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Assay Detail

CHEMBL5100002

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to recombinant human CaMK2alpha 6x hub domain (345 to 475 residues) Trp403 residue assessed as inhibition of intrinsic tryptophan fluorescence by Trp flip assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Exploring the NCS-382 Scaffold for CaMKIIα Modulation: Synthesis, Biochemical Pharmacology, and Biophysical Characterization of Ph-HTBA as a Novel High-Affinity Brain-Penetrant Stabilizer of the CaMKIIα Hub Domain.
Tian Y, Shehata MA, Gauger SJ, Veronesi C, Hamborg L, Thiesen L, Bruus-Jensen J, Royssen JS, Leurs U, Larsen ASG, Krall J, Solbak SMØ, Wellendorph P, Frølund B.
J Med Chem (2022) 65 : 15066 -15084
PubMed 36346645 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 5.74 5.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1031 5-HYDROXY-DICLOFENAC 1 5.74
CHEMBL5170093 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1031 5-HYDROXY-DICLOFENAC IC50 = 1810.0 nM 5.74
CHEMBL5170093 IC50 = 452000.0 nM -